???????SMMC?7721????? - ????????
?????????pUC18?DA. ?pUC18?DJ?1? 3.5 ?????????????. ?100 JL ????E. coli DH5a ???15 JL. pUC18?DA ?pUC18?DJ?1 ?????? ...
?????????????????????????DH5a ??????????????. ??????PCR??????????????. 5 ?. ????????TaCLC-e-3AL ?????????????. 837. Page ... UntitledPCR ????????????,??. ????T ??????????DH5a, ??PCR ??,?. ????????????? 1.2.2 PCR ?????????hTR ????,? ... (12) ??????20?. ?. 1800.00. DH5a-T1?????. BC121-02. 100ulx20. ?. 420.00. D-3 ????. BC122-01 dam-/dcm-?????. 100ulx20. ?. 420.00. ??. ????. ??. ?????????????????????? - Virologica Sinicacoli DH5a(Tiangen. ??)?????33 ?L, ??30 min, 42????. 90 s, ??2 min, ??800 ?L LB ?????, 37?. ????45 min ??????LB ??????,. 37 ... ??????claudin-4 cDNA ???????? - ????????????????????DH5a???????????????????????????. ?????EditSeq???????????????????????????? ... ?????DJ?1 ???????????Construction of Eukaryotic ...?????DH5a ????????????DH5a. ?????LB ???????????????. ???????pK18??mhbM ????E.coli S17?1. ????????S17 ... ??TaCLC-e-3AL ?????????????... ?????E. coli DH5a. ?,?????LB?????????,??5mL LB??????(?????????. ??????),?37????,220r/min ??????????? ... ????????(DH5a),??????????5???. ????LB ?????(?50ug ... ??????DH5g??????????????. ??DNA ??????????? ... ??????P3 ?3????????????????Identification ...??DH5a???????. ??????????????????????. ????????????????????? 1.4 ?????????. pACT1-GFP?????? ... (12)??????16?????? ?????????????DH5a?10?l?. ???100 tA??????????30 rain???. 42?90 s???????5 min? ... LIST OF APPLICANTS CALLED FOR SCREENING INTERVIEW ...269. JAYAKUMAR M. MANIVEL. 10/04/1980 M. SC. PALLAN. 1/124, MUNGILERI, MALLUR, SALEM. -/T. 270. RAMESH C. CHIDAMABARAM 10/04/1990 M. TNEBES... TOWN,. SALEM-1. 157. 2013. S.K.S.ANGURAJ,. S/O.A.S HANMUGAM,. 92.A.THUMBAL MAINRD ... T.D.SURESH. S/O T.V.DHAMODARAN. 69.E, CHAIRMAN SADAGOPAN. ST,. SALEM 36001.
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