??????????????????????????? - OPAC
N ORF?34 -735 nt?702??????????????4??cluster??~?????????????????1000???????. ???????????
???????????? ?????????????????????? total RNA ??????High Capacity cDNA Reverse. Transcription kit (Life Technologies ?) ????????????Apob mRNA ?????. StepOne ... Osaka University Knowledge Archive : OUKA... ??????????????. ??????????????DNA???RNAP?????? ?0022?. ?????????3?????????????? ... ?? 18 ?? ???????? DNA ????????????? ...RT-PCR ??????????? SuperScriptII Reverse Transcriptase (Invitrogen) ????????. ???? total RNA ? 1 ?g ?????????? (25 ? for 10 ... ?? Aspergillus oryzae ???? ?????????? KpeA ? ...DNase?RT. Grade?Nippon Gene????????oligo(dT)???????????SuperScriptTM III Reverse. Transcriptase(Invitrogen)????????? kpeA ? 5 ... ?????????????????????????? ??????????? cDNA?????????? cDNA?. ?? mRNA ???????? ... RNA??? Omuniscript reverse transcriptase (????)? oligo d(T) ... GC-rich ?????? UPF1 ??? mRNA ?????????? ? ...???? Total RNA ? PrimeScript RT Master Mix?Takara, Shiga, Japan???????. ? cDNA ??????????????????????? SYBR Premix Ex Taq II? ... ??????????????RNaseH ????????HIV-1 ...????????????????????????????? RNaseH ???????????. ??????? ??????????????????????????? ?????????????????????????????????????? cDNA ?????????? cDNA ?. ??mRNA ???????? ... RNA ???Omuniscript reverse transcriptase??????? oligo d(T)12 ... Untitled - ??????????????cDNA?????????????????????????? 8kbp??????cDNA???filamin A?FLNA??filamin B?FLNB??ARPE?19????. ??????? ... JP 2020?530498 A 2020.10.22 10 (57)???? ????????? ...???????????????????????????????????????????. ????????????????????????????????? ... ??????????????????????? ?? ?? 25 ? ...???? ?? ??????? ?????? ???????? ??????. ??????? ???? ? ? ??????? ?????????? ?. ??????????? ?????????????????????. 9. 2?1???????????????. 9. 2?2?????????????????.
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