Guidelines for the use of cell lines in biomedical research
We analyzed the impact of each method on the cell adsorption rate onto ConA beads using MCF-7 cells. Based on these optimization experiments ...
Optimising single-cell gene expression profiling of circulating tumour ...Using this method, we tested the change of the cell properties of human cancer breast epithelial cell line, MCF-7 ... After thawing initially frozen cells ... D3.3 Report on characterization in vitro human/animal mammary ...An aliquot of soluble protein was obtained by one cycle of freezing/thawing the cells, vortexing (30 sec), and centrifuging at 12,000 ? g (1 min) ... Guidance Document on Good In Vitro Method Practices (GIVIMP)Total cell homoge- nate was centrifuged at 15,000 3 g for 10 min, and the supernatant was collected and used for enzyme activity assay or immunoblot analysis.
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