Td corrigé traditional poster - ismrm pdf

traditional poster - ismrm

Des rapports du sujet à une langue et de son action sur elle. Logique du patois ...... p 306-307 : Commission d'examen des oeuvres en patois saintongeais. « En tout cas, à ...... Le ticket de chocolat ..... Qu'est-ce que le « cochet » ? Bulletin de ...




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TRADITIONAL POSTER
Give the Dog a Bone
Hall B Monday 14:00-16:00
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/784_3369.pdf'))%7b%7d"  784. Preliminary In-Vivo Bone Quantification Results Using MR and PQCT
Victor Rakesh Lazar1, Gary P. Liney2, David J. Manton1, Peter Gibbs1, Martin Lowry1, Celia L. Gregson3, Joern Rittweger4, Sue Steel5, Chris Langton6, J H. Tobias3, Lindsay W. Turnbull1
1Centre for MR Investigations, University of Hull, Hull, North Humberside, United Kingdom; 2Radiotherapy Physics, University of Hull, Hull, North Humberside, United Kingdom; 3Academic Rheumatology, University of Bristol, Bristol, United Kingdom; 4Excercise and Sports Medicine, Manchester Metropolitan University, Manchester, United Kingdom; 5Centre for Metabolic Bone Disease, Hull Royal Infirmary, Hull, North Humberside, United Kingdom; 6School of Physical and Chemical Sciences, Queensland University of Technology, Australia
Bone quantification is commonly measured using DEXA and pQCT. Research in MRI and MRS have shown promising potential in the quantification of bones. Our work was based on using these ideas in a clinical setting on individual patients. The work was involved in a High Bone Mass (HBM) study program to identify individuals affected with a genetic condition of LRP–5. pQCT data was collected from 169 individuals from the HBM study. 43 people were selected for MRI and MRS acquisition from the total pQCT population. Preliminary results from these investigations have been explained in this abstract.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/785_3321.pdf'))%7b%7d"  785. Software Tools for MR and PQCT Bone Quantification
Victor Rakesh Lazar1, Gary P. Liney2, David J. Manton1, Peter Gibbs1, Martin Lowry1, Celia L. Gregson3, Joern Rittweger4, Sue Steel5, Chris Langton6, J H. Tobias3, Lindsay W. Turnbull1
1Centre for MR Investigations, University of Hull, Hull, North Humberside, United Kingdom; 2Radiotherapy Physics, University of Hull, Hull, North Humberside, United Kingdom; 3Academic Rheumatology, University of Bristol, Bristol, United Kingdom; 4Excercise and Sports Medicine, Manchester Metropolitan University, Manchester, United Kingdom; 5Centre for Metabolic Bone Disease, Hull Royal Infirmary, Hull, United Kingdom; 6School of Physical and Chemical Sciences, Queensland University of Technology, Australia
Peripheral Quantitative Computed Tomography (pQCT) and Dual Energy X-Ray Absorptiometry (DEXA) are the current gold standards for the measurement of bone density and structure, in the research and clinical setting respectively. However, Magnetic Resonance Imaging (MRI) and unsuppressed 1H Magnetic Resonance Spectroscopy (MRS) can also offer several advantages including the ability to quantify bone marrow content and structure. In-house software was developed to process and evaluate cortical and trabecular bone structure, marrow composition and vertebrae segmentation using data from MRI/MRS and structural details from pQCT.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/786_1857.pdf'))%7b%7d"  786. Characterization of Bone Explants by Magnetic Resonance Microscopy
Ingrid E. Chesnick1, Carol B. Fowler1, Francis A. Avallone2, Kimberlee Potter1
1Department of Biophysics, Armed Forces Institute of Pathology Annex, Rockville, MD, United States; 2Department of Genitourinary Pathology, Armed Forces Institute of Pathology, Washington, DC, United States
MRI studies of tissue engineered constructs prior to implantation clearly demonstrate the utility of the MRI technique for monitoring the bone formation process. However, in our studies of osteoblast-seeded scaffolds, implanted on the chorioallantoic membrane of a chick embryo, we have found that the presence of angiogenic vessels and fibrous tissue around the implant can confound MRI findings of bone deposition. On-going studies support the use of targeted contrast agents for studying mineral deposition and blood vessel infiltration in tissue engineered scaffolds post-implantation.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/787_1182.pdf'))%7b%7d"  787. Performance of 7T µMRI-Based Virtual Bone Biopsy for Structural and Mechanical Analysis at the Distal Tibia
Yusuf Abu Tayeb Bhagat1, Chamith S. Rajapakse1, James H. Love1, Michael J. Wald1, Jeremy F. Magland1, Alexander C. Wright1, Hee Kwon Song1, Felix W. Wehrli1
1Laboratory for Structural NMR Imaging, University of Pennsylvania, Philadelphia, PA, United States
The detection of subtle microstructural trabecular bone (TB) alterations such as the conversion of plates to rods requires adequate signal-to-noise ratio (SNR), which governs achievable spatial resolution and scan time. Increased SNR may enhance detection sensitivity for microstructural changes in treatment studies. Here, the reproducibility of TB quantitative parameters was investigated using a new 3D fast-spin-echo technique at 7.0T. The imaging and analysis protocol is shown to provide highly reproducible measures of scale, topology and mechanical parameters related to TB microstructure. Performance improvements relative to earlier work are attributed to enhanced SNR, motion control and correction, and improved registration techniques.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/788_2722.pdf'))%7b%7d"  788. Studying the Effect of Different Biomaterials on Healing Process in Bone Injury Model Using Microscopic MRI and Micro CT
May Abdel Hamid Taha1, Sarah L. Manske2, Erika Kristensen3, Jaymi T. Taiani4, Roman Krawetz5, Ying Wu, Steven K. Boyd6, John Robert Matyas7, Derrick E. Rancourt5, Jeffery F. Dunn1
1Radiology, University of Calgary, Calgary, Alberta, Canada; 2Kinesiology; 3Mechanical and Manufacturing Engineering; 4 Medical Sciences; 5Departments of Oncology, Biochemistry & Molecular Biology and Medical Genetics; 6Mechanical and Manufacturing Engineering; 7Comparative Biology and Experimental Medicine in Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada
The objective of this study was to investigate the effect of different biomaterials on bone healing in vivo in a mouse model of bone injury. These materials; matrigel, purecol gel and hydroxyapatite (HA) are potential matrices to support stem cells. Optimized in vivo MR microscopy and micro computed tomography were used to assess fracture repair. In addition, MRI images and µCT scans were compared at the same time point, to show the difference between them in revealing the actual stage of healing.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/789_5364.pdf'))%7b%7d"  789. Traditional Bone Structural Parameters on Different Resolutions in Magnetic Resonance Imaging
June-Goo Lee1,2, Gyunggoo Cho1, Youngkyu Song1, Jong Hyo Kim2, Namkug Kim3
1Division of Proteome Research/Bio-Magnetic Resonance Research Center, Korea Basic Science Institute, Cheongwon-Gun, Chungcheongbuk-Do, Korea, Republic of; 2Interdisciplinary Program in Radiation Applied Life Science, Seoul National University College of Medicine, Seoul, Korea, Republic of; 3Department of Radiology, Asan Medical Center, University of Ulsan, Seoul, Korea, Republic of
Our study focused on the development of robust image processing algorithm on low resolution ¼-MR bone image
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/790_5829.pdf'))%7b%7d"  790. Advanced Image Analysis Techniques of New High-Resolution Images of the Proximal Femur in the Presence of Red and Yellow Bone Marrow Using Local Bone Enhancement Fuzzy Clustering
Jenny Folkesson1, Julio Carballido-Gamio1, Dimitrios C. Karampinos1, Patrick Koon2, Suchandrima Banerjee2, Eric Han2, Thomas M. Link1, Sharmila Majumdar1, Roland Krug1
1Radiology and Biomedical Imaging, University of California, San Francisco, CA, United States; 2Applied Science Laboratory, GE Heathcare, Menlo Park, CA, United States
With the advent of new MR hardware and pulse sequences it is now possible to image the small trabecular structure of deeper seated regions like the proximal femur with high spatial resolution in a clinically feasible scan time. We employ a novel partial membership bone segmentation technique (BE-FCM) technique that enhances bone segmentation compared to an established dual thresholding method in the presence of signal variations due to different marrow types. We demonstrate that the new image acquisition and analysis framework enables trabecular bone analysis in the deeply situated femoral head, something which has been previously unfeasible in vivo.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/791_1852.pdf'))%7b%7d"  791. Bone Marrow Fat Fraction Mapping in the Proximal Femur in Vivo Using IDEAL Gradient Echo Imaging
Dimitrios C. Karampinos1, Huanzhou Yu2, Ann Shimakawa2, Eric T. Han2, Thomas M. Link1, Sharmila Majumdar1, Roland Krug1
1Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA, United States; 2Applied Science Laboratory, GE Healthcare, Menlo Park, CA, United States
There is some evidence that osteoporosis is associated with increased marrow fat content as well as an accelerated conversion from red marrow to yellow (fatty) marrow with age. In this work, we investigated the marrow fat composition in the proximal femur in vivo using IDEAL gradient echo imaging. 3-point IDEAL FGRE hip images of six healthy subjects were acquired and water-fat separation was performed using multi-peak IDEAL. The average fat fraction and standard deviation were determined in three different regions of interest (femoral head, greater trochanter and neck). Significant differences in marrow fat content were identified between the three regions for all subjects.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/792_4625.pdf'))%7b%7d"  792. 1H MRS to Detect Biochemical Degenaration of the Vertebral Bone Marrow in Gaucher Disease
Simona Ortori1, Michela Tosetti2, Marzio Perri3, Margherita Marchetti1, Gabriele Caproni1, Laura Biagi2, Mirco Cosottini4, Virna Zampa1, Giuliano Mariani3, Carlo Bartolozzi1
1Divisione di Radiologia Diagnostica ed Interventistica, Azienda Ospedaliera Universitaria Pisana, Pisa, Italy; 2MR Laboratory, Stella Maris Scientific Institute, Pisa, Italy; 3Divisione di Medicina Nucleare, Azienda Ospedaliera Universitaria Pisana, Pisa, Italy; 4Dipartimento di Neuroscienze, Università di Pisa, Pisa, Italy
Gaucher disease is the most prevalent inherited, lysosomial storage disease and results in a deficient level of activity of ²-glucocerebrosidase, a membrane-bound lysosomal enzyme. This deficiency leads to accumulation of the lipid glucocerebroside in the lysosomes of monocytes and macrophages, called Gaucher cells. The symptoms and pathology of Gaucher disease result from the accumulation of Gaucher cells in various organ system, including vertebral bodies. To evaluate the biochemical process underlying the infiltration of Gaucher cells, 1H-MRS has been acquired on vertebral bone marrow in patients affected by Gaucher disease, highlighting a significant reduction of fat content of any age range
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/793_5020.pdf'))%7b%7d"  793. Characterization of Trabecular Orientation in Chicken Femur by Multi-Directional SPENT (Sub-Pixel Enhancement of Non-Uniform Tissue)
Bailiang Chen1, Bernard Siow2, David Carmichael1,3, Freddy Odille2, Roger Ordidge1, Andrew Todd-Pokropek1
1Medical Physics and Bioengineering, University College London, London, United Kingdom; 2Centre for Medical Image Computing, University College London, London, United Kingdom; 3Institute of Neurology , University College London, London, United Kingdom
The recently proposed SPENT sequence can provide direction specific information based on the sub-voxel structural uniformity of a sample. Analogous to diffusion tensor imaging, given a voxel with a local anisotropic structure (e.g. trabecular bone), it is possible to characterize the orientation of sub-pixel micro-structure by applying SPENT with multiple directions. A 6-direction SPENT series was applied to a chicken femur head in order to characterize its trabecular bone orientation by reconstructing a 2D tensor in each voxel. Both tensor statistics and eigensystems were computed and showed good qualitative agreement with data from a subsequent micro-CT acquisitions.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/794_4371.pdf'))%7b%7d"  794. The Effect of Freezing on Measurements of Trabecular Bone Structure Based on NMR Spectroscopy
Viktoria Prantner1, Hanna Isaksson1, Johanna Närväinen2, Eveliina Lammentausta3, Olli HJ Gröhn2,4, Jukka S. Jurvelin1
1Department of Physics, University of Kuopio, Kuopio, Finland; 2A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland; 3Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland; 4Biomedical Imaging Unit, University of Kuopio, Kuopio, Finland
NMR is a potential tool for the assessment of trabecular bone structure. Since trabecular bone provides a negligible NMR signal, the indirect evaluation of the trabecular bone structure is based on the analysis of water and fat components in the bone marrow. Earlier studies have revealed that freezing affects the bone marrow structure, suggesting there may be changes in the molecular structure. The aim of the current study is to investigate the effect of freezing on trabecular bone and bone marrow, as assessed by NMR spectroscopy.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/795_5667.pdf'))%7b%7d"  795. Performance of Two Spin-Echo Sequences for Quantitative Structure Analysis of Trabecular Bone
Michael Jeffrey Wald1, Jeremy Francis Magland1, X. Edward Guo2, Felix Werner Wehrli1
1Laboratory for Structural NMR Imaging, University of Pennsylvania Medical Center, Philadelphia, PA, United States; 2Bone Bioengineering Laboratory, Columbia University, New York, United States
The performance of two spin-echo based pulse sequences for imaging trabecular bone microstructure are evaluated at 1.5T in seven fixed, cadaveric distal tibia specimens. SNR efficiency and sensitivity of image-derived trabecular bone structural parameters to variations in bone quality as assessed by µCT were investigated. Inter- pulse sequence correlations suggest similar structural sensitivity, while comparisons to µCT reveal good sensitivity but large deviations in absolute values between modalities.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/796_6135.pdf'))%7b%7d"  796. Ultra-Short TE (UTE) Imaging of Skull and a Quantitative Comparison of Skull Images Obtained from MRI and CT
Liya Wang1, Xiaodong Zhong2, Longjiang Zhang3, Diana Tiwari1, Hui Mao1
1Department of Radiology and Emory Center for Systems Imaging, Emory University School of Medicine, Atlanta, GA, United States; 2MR R&D Collaborations, Siemens healthcare, Atlanta, GA, United States; 3Department of Radiology, Jinlin Hospital and Nanjing University College of Clinical Medicine, Nanjing, Jiangsu, China
This study provided a quantitative evaluation of skull images obtained using UTE MRI and a direct comparison to those from CT. The skull thickness measured from UTE images showed good agreement with those obtained from CT images in different slices. There is also a good correlation between the thickness measurements obtained from CT and UTE images. Signal intensity based evaluation showed that there is no statistical difference between UTE and CT images in outer, inner layer and diploe of the skull. The comparison of bone UTE MRI and CT of skull suggests that UTE images match closely with CT images.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/797_4563.pdf'))%7b%7d"  797. MR Imaging Detects Impaired Angiogenesis and Trabecular Bone Formation During Endochondral Bone Growth Mediated Through PKBalpha/Akt1 in Gene Dosage Dependent Manner
Katrien Vandoorne1, Jeremy Magland2, Vicki Plaks1, Inbal E. Biton3, Amnon Sharir4,5, Elazar Zelzer4, Felix Wehrli6, Brian A. Hemmings7, Alon Harmelin3, Michal Neeman1
1Biological Regulation, Weizmann Institute, Rehovot, Israel; 2Department of Radiology, University of Pennsylvania Health System, Philadelphia, PA, United States; 3Veterinary Resources, Weizmann Institute, Rehovot, Israel; 4Molecular Genetics, Weizmann Institute, Rehovot, Israel; 5The Laboratory of Musculoskeletal Biomechanics and Applied Anatomy, Koret School of Veterinary Medi, Hebrew University of Jerusalem, Rehovot, Israel; 6Department of Radiology, University of Pennsylvania Health System, Philadelphia, PA, Israel; 7Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
Since infiltration of the newly formed blood vessels is required for endochondral bone formation, and PKBalpha/Akt1 mediates intracellular signaling of angiogenesis, we postulated that a vascular deficiency at the site of the long bones could contribute indirectly to impaired bone development in PKBalpha/Akt1 deficient mice. Our study demonstrated using macromolecular DCE-MRI in vivo and ex vivo µCT and µMRI, vascular and bone developmental defects in PKBalpha/Akt1 null mice, and remarkably also in heterozygous mice, lacking a single copy of the gene.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/798_4080.pdf'))%7b%7d"  798. Water and Fat Suppressed Proton Projection MRI (WASPI) Study on Bone Specimens After Proton-Deuteron Exchange
Haihui Cao1,2, Jerome L. Ackerman, 2,3, Guangping Dai, 2,3, Mirko Hrovat4, Melvin J. Glimcher, 2,5, Yaotang Wu, 2,5
1Department of Orthopedic Surgery, Children's Hospital , Boston, MA, United States; 2Harvard Medical School, Boston, MA, United States; 3Department of Radiology, Massachusetts General Hospital, Boston, MA, United States; 4Mirtech, Inc, Brockton, MA, United States; 5Department of Orthopedic Surgery, Children's Hospital, Boston, MA, United States
Questions have arisen as to the nature of the molecular species giving rise to the short-T2 proton signal in Water- and fat-suppressed proton projection MRI (WASPI), a noninvasive means to image bone. In this study we use deuterium exchange to identify the source of proton signal in WASPI.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/799_7283.pdf'))%7b%7d"  799. Quantification of Bound and Mobile Water in Human Cortical Bone by 1H and 2H Magnetic Resonance
Henry H. Ong1, Alexander C. Wright1, Felix W. Wehrli1
1Laboratory for Structural NMR Imaging, Department of Radiology, University of Pennsylvania School of Medicine, Philadelphia, PA, United States
Magnetic resonance is a powerful tool for non-destructive study of bone water, which can provide insight into bone micro- and nanostructure. However, the MR signal of bone is comprised of several proton populations including collagen-associated water, and water within the pore space (Haversian and lacuno-canalicular system). We hypothesize that water in pores is predominantly free and water in the bone matrix is predominantly associated with collagen. Using 2H exchange and inversion recovery experiments, we estimated porosity in human cortical bone and found it to agree with micro-CT based volumetric measurements with a significant fraction being collagen-associated.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/800_3900.pdf'))%7b%7d"  800. MR Spin Behavior During RF Pulses: T2 Vs. T2' Relaxation
Michael Carl1, Nikolaus Szeverenyi2, Mark Bydder2, Eric Han1, Graeme Bydder2
1GE Healthcare, Waukesha, WI, United States; 2University of California, San Diego
We investigated the behavior of the MR magnetization vector during RF pulses in the presence of rapid transverse relaxation caused by either amplitude loss or spin dephasing. We found that different tissues with the same T2* may generate different responses to RF pulses, dependent on whether the relaxation is dominated by a homogeneously or inhomogeneously broadened linewidth and RF optimization may hence require explicit knowledge of the intrinsic T2 and T2* of the tissue.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/801_2851.pdf'))%7b%7d"  801. Multi-Modality Imaging of Bone Marrow Edema-Like Lesions and Associated Cartilage in Osteoarthritic Patients
Daniel Kuo1, Joseph Schooler1, Janet Goldenstein1, Sarmad Siddiqui1, Swetha Shanbhag1, Jean-Baptiste Pialat1, Andrew Burghardt1, Sharmila Majumdar1, Michael Ries2, Galateia Kazakia1, Xiaojuan Li1
1Musculoskeletal Quantitative Imaging Research (MQIR), Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA, United States; 2Department of Orthopaedic Surgery, University of California, San Francisco, San Francisco, CA, United States
Osteoarthritis (OA) is a complex disease that affects both cartilage and bone. Bone marrow edema-like lesions (BMEL) are important in studying OA, but knowledge about them is limited. In this study, we take a multi-modality imaging approach, examining MR T1ñ and T2 values of BMEL-associated cartilage plus structure and composition of BMEL tissue using high resolution peripheral quantitative computed tomography (HR-pQCT) imaging and Fourier transform infrared (FTIR) spectroscopy. Our results indicate that regions of BMEL are associated with more advanced cartilage degeneration and that there is a localized imbalance in bone formation and mineralization specific to BMEL regions.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/802_5786.pdf'))%7b%7d"  802. Perfusion Abnormalities of Bone Marrow Edema-Like Lesions in Knees with Anterior Cruciate Ligament Injury Using Dynamic Contrast-Enhanced MRI
Jin Zuo1, Sharmila Majumdar1, Xiaojuan Li1
1Radiology and Biomedical Imaging, Univ. of California, San Francisco, San Francisco, CA, United States
Anterior cruciate ligament (ACL) tear is a common knee injury, and is a risk factor of post-traumatic osteoarthritis (OA). The disease is frequently associated with bone marrow edema-like (BMEL) lesions which exhibit as an area of high signal intensity in T2-weighted, fat-saturated fast spin echo MR images. BMEL is also commonly seen in OA and has been associated with disease progression and pain in OA. However, the knowledge on the pathophyisiology and significance of BMEL in ACL-injured knees is very limited. Dynamic contrast enhanced MRI (DCE-MRI) can probe bone marrow and subchondral bone perfusion as well as fluid dynamics. Impaired perfusion in bone may lead to cartilage degeneration. A recent study showed bone marrow abnormalities were associated with BME in OA. The aim of this study is to apply DCE MRI to evaluate bone marrow perfusion in patients with ACL tears, and to compare the perfusion patterns between BMEL region and normal appearing bone marrow region.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/803_1371.pdf'))%7b%7d"  803. The Influence of Running on Patellar Water Content and Bone Marrow Edema in Females with and Without Patellofemoral Pain
Kai-Yu Ho1, Houchun H. Hu2, Krishna S. Nayak2, Patrick M. Colletti3, Christopher M. Powers1
1Division of Biokinesiology and Physical Therapy, University of Southern California, Los Angeles, CA, United States; 2Department of Electrical Engineering, University of Southern California, Los Angeles, CA, United States; 3Department of Radiology, University of Southern California, Los Angeles, CA, United States
The purpose of this study was to investigate the influence of running on patellar water content and bone marrow edema (BME) in individuals with and without patellofemoral pain (PFP). Using the IDEAL protocol, two subjects with PFP and 2 pain-free controls were evaluated. Each subject underwent a pre-running MR scan, a 40-min moderate effort running, and a post-running MR scan. Our data showed that in persons with PFP, content and volume of local BME increases post-running. Additionally, the PFP subjects demonstrated increased water content of the bone marrow region post-running while the controls showed no changes in water content.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/804_5728.pdf'))%7b%7d"  804. Using 18F NaF PET/CT to Image Increased Bone Activity in Patellofemoral Pain: Correlation with MRI
Christine Elizabeth Draper1, Michael Fredericson1, Thor F. Besier1, Gary S. Beaupre2, Scott L. Delp1, Andrew Quon1, Garry E. Gold1
1Stanford University, Stanford, CA, United States; 2VA Palo Alto Health Care System, Palo Alto, CA, United States
Articular cartilage deterioration is associated with the development of osteoarthritis. Cartilage health depends upon the integrity of the underlying subchondral bone and there may be abnormalities in bone metabolic activity that accompany structural defects in bone and cartilage. 18F NaF PET/CT enables bone metabolic activity to be visualized. We compared metabolic abnormalities detected using PET/CT with structural defects seen using MRI. We found that regions of increased bone metabolic activity do not always correlate with cartilage damage or bone marrow edema, indicating that 18F NaF PET/CT may image bone abnormalities prior to the development of structural damage seen using MRI.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/805_6327.pdf'))%7b%7d"  805. Improved Fat-Suppression for Unspoiled GRASS Imaging of the Knee Using Multi-Peak IDEAL Chemical Shift Fat-Water Separation
Richard Kijowski1, Catherine Debra Hines2, Huanzhou Yu3, Scott Brian Reeder1,2
1Radiology, University of Wisconsin, Madison, WI, United States; 2Medical Physics, University of Wisconsin, Madison, WI, United States; 3GE Healthcare, Applied Science Laboratory, Menlo Park, CA, United States
This study was performed to demonstrate improvements in the quality of fat-suppression for unspoiled GRASS imaging of the knee using multi-peak fat spectral modeling and IDEAL fat-water separation. An IDEAL-GRASS sequence was performed at 3.0T on the knees of 10 asymptomatic volunteers. The IDEAL-GRASS images were reconstructed using a single-peak method and a multi-peak method that more accurately models the NMR spectrum of fat. Multi-peak IDEAL-GRASS had significantly greater (ppre contrast, however detailed ROI analysis shows differences in sub-auditory areas.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1180_4859.pdf'))%7b%7d"  1180. Functional Imaging of Observation of Action in Elite Archers Using Video of Western-Style Archery Task
Hui-jin Song1, Joo-hyun Kim1, Jeehye Seo1, Moon-jung Hwang2, Young-ju Lee2, Kyung Jin Suh3, Sung Woo Kim3, Young Hwan Lee4, Dong Soo Yoo5, Yongmin Chang1,6
1Medical & Biological Engineering, Kyungpook National University, Daegu, Korea, Republic of; 2GE healthcare, Seoul; 3Dongguk University, Gyungju; 4Radiology, College of Medicine, Catholic University, Daegu; 5Radiology, College of Medicine, Dankook University, Chunan; 6Diagnostic Radiology, Kyungpook National University, Daegu, Korea, Republic of
Although the mirror neuron system has been extensively studied, no functional imaging data are currently available to gain insight in the possible difference of the mirror system between experts and novices. Therefore, the aim of the present study is to investigate the differences of activation in the mirror neuron system during viewing tool use familiar to experts between expert archers and novice subjects. Our results demonstrated that expert archers showed strong activation in the mirror neuron system during viewing videos of Western-style archery relative to inexpert control subjects. Taken together, our data consistent with previous reports suggest that human mirror neuron system could contain representations of tool use and expand motor repertoire with tool use experiences.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1181_1173.pdf'))%7b%7d"  1181. Localization of the Hand Motor Area Using BOLD and ASL FMRI
Marco Pimentel1, Pedro Vilela2, Inês Sousa3,4, Patricia Figueiredo3
1Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Lisbon, Portugal; 2Imaging Department, Hospital da Luz, Lisbon, Portugal; 3Instituto Superior Técnico, Lisbon, Portugal; 4Healthcare Sector, Siemens, S.A., Portugal
Previous studies have shown that ASL-based fMRI exhibits better spatial specificity than the most commonly used BOLD contrast. Here, we compared the localization of the hand motor area obtained by simultaneous ASL-BOLD fMRI and standard BOLD fMRI at 3T with well established anatomical landmarks, in a group of 15 healthy subjects. Our results indicate that the localization of the hand motor area obtained using ASL fMRI is significantly less variable and closer to the hand motor cortex anatomical landmarks than the one produced by BOLD fMRI. This supports the notion that ASL may more accurately localize brain activation than BOLD.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1182_3927.pdf'))%7b%7d"  1182. Understanding Consciousness from Information and Integration Within the Thalamocortical System
Xiaolin Liu1, Jingsheng Zhou2, Anthony G. Hudetz3, Shi-Jiang Li1
1Biophysics, Medical College of Wisconsin, Milwaukee, WI, United States; 2Rehabilitation Department, Xuanwu Hospital Capital Medical University, Beijing, China; 3Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United States
Understanding the neural mechanisms of consciousness requires identification of the nature of contributions from each of the potential neural correlates, which together generate a complete cognitive experience. We examined the specific and nonspecific thalamic connections in the brain based on the neuroanatomical findings implicating their respective functional roles in sustaining information and integration, which are essential to consciousness. Our results endorse the view that the thalamocortical system is essential to consciousness, and support the hypothesis that the nonspecific thalamic connections largely reflect brain regions that are responsible for information integration, potentially sustaining various awareness functions.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1183_6781.pdf'))%7b%7d"  1183. Examining Structure and Function in a Cognitive Task
Jeffrey Thomas Duda1, Corey McMillan, Murray Grossman, James Gee
1Bioengineering, University of Pennsylvania, Philadelphia, PA, United States
Structure and function are examined in the language network with DT-MRI and BOLD fMRI during a cognitive task. Activated cortical regions are identified and used to determine activation levels in each subject. Additionally, the regions are used to identify fiber tracts of interest. Canonical correlation analysis is used to identify correlations between functional activation and average fractional anisotropy in the fiber tracts. For each correlation found, the highest weightings are found for cortical regions and a tract that connects to that region.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1184_3382.pdf'))%7b%7d"  1184. Effects of FMRI Acoustic Scanner Noise on Neural Processing Networks During Task Performance and Rest
Dave Langers1, Pim van Dijk1
1Otorhinolaryngology, University Medical Center Groningen, Groningen, Netherlands
Scanner acoustic noise may detrimentally affect stimulus/task-evoked neural responses in fMRI. This has been reported for the unimodal and associative auditory systems, but also for the default mode network and other brain systems.
In the current experiment, the effects of scanner noise in resting state fMRI are studied. We find that similar independent components may be extracted with and without background scanner noise, both during active and resting states. However, the overall strength, spatial extent, and temporal dynamics of various neural components are affected by the presence of background noise. Our results both corroborate and extend previous findings in literature. More detailed specific findings for various brain systems will be presented.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1185_262.pdf'))%7b%7d"  1185. Neural Correlates of Feigned Hearing
Bradley McPherson1, Wayne Wilson2, David Copland3,4, Katie McMahon5
1Division of Speech and Hearing Sciences, Hong Kong University, China; 2Division of Audiology, University of Queensland, Australia; 3Centre for Clinical Research, University of Queensland, Australia; 4School of Health and Rehabilitation Sciences, University of Queensland, Australia; 5Centre for Magnetic Resonance, University of Queensland, Brisbane, Queensland, Australia
Can we use patterns of brain activity to detect when someone is feigning a hearing loss? To answer this question, we asked 15 adult participants to respond to pure tones and simple words correctly, incorrectly, randomly, or with the intent to feign a hearing loss.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1186_2868.pdf'))%7b%7d"  1186. An FMRI Study of Memory Performance in Type 2 Diabetes Mellitus: A Twin Study
Amanda Wood1, Jian Chen2,3, Thanh G. Phan2, Kimberlea Cooper2, Stacey Litras2, Srikanth Velandai2
1Developmental and Functional Brain Imaging,Critical Care and Neuroscience, Murdoch Childrens Research Institute, Melbourne, VIC, Australia; 2Stroke and Ageing Research Group, Department of Medicine, Monash University, Melbourne, VIC, Australia; 3Developmental and Functional Brain Imaging,Critical Care and Neuroscience , Murdoch Childrens Research Institute, Melbourne, VIC, Australia
Type 2 Diabetes mellitus (DM) is linked to a greater risk of dementia, but the underlying mechanisms and brain regions involved are unknown. We conducted a co-twin (DM/non DM) case-control study of fMRI activation during a visual memory task. Non DM twins showed greater activation of temporal, parietal and occipital cortices suggesting involvement of these areas in DM pathology.
Animal fMRI
Hall B Wednesday 13:30-15:30
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1187_2615.pdf'))%7b%7d"  1187. Increased Sensitivity to the BOLD-FMRI Signal Response During Electrical Forepaw Stimulation in Mice Using a Cryogenic RF Probe
Christof Baltes*1, Simone Bosshard*1, Thomas Mueggler1,2, Markus Rudin1,3
1Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland; 2Pharmaceutical Research Neuroscience, F. Hoffmann-La Roche Ltd., Basel, Switzerland; 3Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland
BOLD-fMRI in mice using electrical forepaw stimulation was performed with a cryogenic transceive RF probe and a room-temperature receive-only surface coil. The effect of the increased sensitivity of the cryogenic probe on detecting BOLD responses was analyzed. In fMRI experiments, a gain in image SNR and in temporal SNR of a factor of 3.10 and 1.77 was found, respectively. As further optimization parameter adjusting the thermal shield temperature of the cryogenic probe allows for altering baseline perfusion and accordingly BOLD responses. Cryogenic cooling reduces BOLD signal variations by a factor of 1.59 and therefore increases the statistical power of fMRI.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1188_552.pdf'))%7b%7d"  1188. Investigating Color Vision Using FMRI: Rodent Vs Primate
Andy Paul Salzwedel1, Matt Mauck2, James Kuchenbecker3, Chris Pawela1, James Hyde1, Maureen Neitz3, Jay Neitz3
1Biophysics, Medical College of Wisconsin, Milwaukee, WI, United States; 2Department of Ophthalmology, Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI, United States; 3Ophthalmology, University of Washington, Seattle, WA, United States
In a comparative study, the visual pathways of two different animal models (rodent vs primate) were probed using high field strength (9.4T) fMRI. The primary goal of this research was to assay the color vision systems of these two species in hope of demonstrating the evolutionary homology thereof. Here we present several techniques that combine to form a unique overall method for probing this pathway; pharmacological intervention (AP4), precession fMRI compatible LED based stimuli, and intra-brain controls.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1189_6536.pdf'))%7b%7d"  1189. Detectability of the BOLD Signal
Jozien Goense1, Hellmut Merkle2, Nikos Logothetis1,3
1Department of Physiology of Cognitive Processes, Max-Planck Institute for Biological Cybernetics, Tuebingen, Germany; 2Laboratory of Functional and Molecular Imaging, NINDS, NIH, Bethesda, MD, United States; 3Division of Imaging Science and Biomedical Engineering, University of Manchester, Manchester, United Kingdom
The BOLD signal is a weak signal, and hence if no BOLD signal is found in an area this does not necessarily mean there is no neural activity in that area. Signal dropout, artifacts, instability, physiological noise, RF-coil inhomogeneity etc. can all reduce the SNR locally leading to decreased detectability of the BOLD signal Here we illustrate that calculation of the spatial distribution of the detection for a given set of experimental conditions allows us to estimate the confidence by which absence of an fMRI signal can be interpreted as an absence of neural activity.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1190_3508.pdf'))%7b%7d"  1190. BOLD FMRI of Anesthetized Baboons
Hsiao-Ying Wey1,2, Jinqi Li1, M. Michelle Leland3, Lisa Jones3, C Akos Szabo4, John W. Roby1, James T. Scribner1,2, Ghzawan M. Kroma2, Peter T. Fox1, Timothy Q. Duong1,2
1Research Imaging Institute, UT Health Science Center at San Antonio, San Antonio, TX, United States; 2Radiology, UT Health Science Center at San Antonio, San Antonio, TX, United States; 3Laboratory Animal Resources, UT Health Science Center at San Antonio, San Antonio, TX, United States; 4Neurology, UT Health Science Center at San Antonio, San Antonio, TX, United States
This study reports a robust anesthetized baboon model for BOLD fMRI studies on a clinical 3T human MRI scanner. BOLD fMRI of visual and somatosensory/motor stimulations in anesthetized baboons were investigated. Comparisons of BOLD fMRI sensitivity were made between isoflurane and ketamine anesthetics with and without paralytics. To our knowledge, this is the first report on baboon BOLD fMRI of visual and somatosensory/motor.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1191_2620.pdf'))%7b%7d"  1191. Using T1 Map to Guide Functional MRI Study of Ipsilateral Somatosensory Cortex in Awake Non-Human Primates
Junjie V. Liu1, Nicholas A. Bock2, Ara Kocharyan1, Julie Mackel1, Afonso C. Silva1
1NINDS, National Institutes of Health, Bethesda, MD, United States; 2Medical Physics, McMaster University, Hamilton, ON, Canada
By combining BOLD fMRI with T1 mapping, here we study the ipsilateral responses in somatosensory cortex of awake marmosets. Our results show a surprising spatial mismatch between contralateral and ipsilateral representations of the same body part.

 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1192_5497.pdf'))%7b%7d"  1192. Fine -Scale Functional Connectivity Network Revealed at High Field (9.4T) Within Somatosensory Cortices of Anesthetized New World Monkeys
Arabinda Mishra1, Baxter P. Rogers1, Barbara Dillenburger1, Kevin Wilson1, Feng Wang1, John C. Gore1, Li Min Chen1
1Radiology & Radiological Science, VUIIS, Nashville, TN, United States
Correlations between resting state BOLD signals in widely distributed brain regions is a key signature of consciously driven mental activity in humans. In this work we attempted to explore if a fine scale functional connectivity can be detected within the anatomically well defined primary somatosensory cortex (SI) at high field and whether the functional connectivity reflects anatomical hierarchical relationships in anesthetized monkeys. We found that functional connectivity exists among anatomically interconnected cortical subregions (areas 3a, 3b, 1 and 2) within SI without the involvement of consciousness (or alertness), and the strengths of the correlation among these subregions reflect the strength of their underlying anatomical connections.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1193_2788.pdf'))%7b%7d"  1193. fMRI Analysis of the Olfactory Responses to Home-Stream Water in Sockeye Salmon
Hiroshi Bandoh1, Ikuhiro Kida2, Hiroshi Ueda1,3
1Division of Environmental Science Development, Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, Japan; 2Integrated Neuroscience Research Team, Tokyo Institute of Pschiatry, Setagaya-ku, Tokyo, Japan; 3Laboratory of Aquatic Ecosystem Conservation, Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Hokkaido, Japan
The odor-information processing involved in olfactory imprinting and homing of the home-stream odor in the central nervous system of salmon has not been completely elucidated. In this study, to investigate this information-processing mechanism, we used BOLD fMRI to measure the response to home-stream water in the olfactory bulb and telencephalon of sockeye salmon. The presence of BOLD signals in the dorsal area of the telencephalon indicated that the odor information for home-stream water was processed in a specific area in the telencephalon of sockeye salmon.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1194_3250.pdf'))%7b%7d"  1194. Evaluation of Functional Deficit and Recovery in the Rat Somatosensory Cortex After Moderate Traumatic Brain Injury Using FMRI
Juha-Pekka Niskanen1,2, Antti Airaksinen1, Jari Nissinen1, Asla Pitkänen1, Olli Gröhn1
1Department of Neurobiology, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland; 2Department of Physics, University of Kuopio, Kuopio, Finland
Traumatic brain injury (TBI) is a major cause of death and disability worldwide. In this study, 10 rats with TBI and 6 sham operated controls were imaged during electrical stimulation of the forepaws before TBI and 1, 2 and 8 weeks after TBI. fMRI with forepaw stimulation was able to reveal functional deficit after TBI in the somatosensory cortex outside of the main lesion and also detect partial sensory recovery 8 weeks after TBI. The results suggest that fMRI could serve as a non-invasive user independent tool to evaluate functional recovery after TBI.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1195_4874.pdf'))%7b%7d"  1195. Complexity in the Spatiotemporal Hemodynamic Response to Sensory Stimulation in the Un-Anesthetized Rat
Christopher James Martin1, Jason Berwick2, Ying Zheng2, John Mayhew2
1Radiation Oncology and Biology, University of Oxford, Oxford, Oxfordshire, United Kingdom; 2University of Sheffield
The aim of this work was to investigate the spatiotemporal changes in hemodynamics that underlie fMRI signal changes in response to stimulus evoked changes in neuronal activity. We used optical imaging spectroscopy in an un-anesthetized rat model, to provide measures of changes in blood volume and oxygenation at higher spatial and temporal resolution than possible with fMRI, without the potentially confounding effects of anesthesia. We found a complex spatiotemporal hemodynamic response function, consisting of both increases and decreases in blood volume and oxygenation as well as oscillatory response components. We discuss the implications of these findings for fMRI.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1196_12.pdf'))%7b%7d"  1196. BOLD Changes in Somatosensory Cortex of Malnourished Rats
R Martin1, R Godinez1, Alfredo O. Rodriguez1
1Departament of Electrical Engineering, Universidad Autonoma Metropolitana Iztapalapa, Mexico, DF, Mexico
Malnutrition is a main public health problem in developing countries. Incidence is increasing and the mortality rate is still high. Functional Magnetic Resonance Imaging (BOLD) was used for mapping brain activity of malnourished rats. The food competition method was applied to a rat model to provoke malnutrition during lactation. The vibrissae-barrel axis was also used due to its advantages for studying structure, function, development and plasticity within the somatosensory cortex. BOLD response changes caused by the trigeminal nerve stimulation on brain activity of malnourished and control rats were obtained at 7T. Results showed a major neuronal activity in malnourished rats.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1197_491.pdf'))%7b%7d"  1197. Temporal Hemodynamic Responses of BOLD FMRI in the Rat Brain Related to Electric Forepaw Stimulation
Dewen Yang1, Zhiyong Xie1, James Goodman1, Anne Burkholder2, Nancy Poy2
1BioImaging COE, Pifizer Global Research & Development, Groton, CT, United States; 2WW Comparative Medicine, Pifizer Global Research & Development, Groton, CT, United States
The neurovascular response to electric forepaw stimulation (EFS) was observed with BOLD signal in the contralateral somatosensory cortex in 11 rats with normal blood gas physiology under alpha-chloralose anesthesia. Serial CBF maps were also acquired during EFS in a subset of these rats. BOLD activation and CBF maps showed elevated blood flow in the contralateral somatosensory cortex at the time of stimulation, after which the regional activation and increases in CBF spread to cortex in the ipsilateral hemisphere.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1198_4430.pdf'))%7b%7d"  1198. Reduced BOLD Response in Mice Lacking Nociceptor Specific Sodium Channels (Nav1.7) Indicates Altered Pain Processing
Simone Claudia Bosshard1, Christof Baltes1, Markus Rudin1,2
1Institute for Biomedical Engineering, ETH Zürich, Zurich, Switzerland; 2Institute of Pharmacology and Toxicology, University of Zurich, Zurich, 8057, Switzerland
Electrical stimulation of the forepaws is a widely used stimulation paradigm in functional magnetic resonance imaging (fMRI). We used transgenic mice lacking the voltage-gated sodium channel Nav 1.7 specifically at the nociceptors (Nav1.7R-/-) to study altered pain sensitivity. Nav1.7R-/-, WT littermates and WT animals of our lab were examined using BOLD fMRI. The detected BOLD signal changes of the Nav1.7R-/- animals were significantly reduced as compared to the two WT groups. This is in line with behavioral data reported for these mice. This proves our method to be a valuable tool to non-invasively study pain processing in mice.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1199_3491.pdf'))%7b%7d"  1199. BOLD Response and Associated Metabolic Changes in the Rat Barrel Cortex Following Sustained Trigeminal Nerve Stimulation
Nathalie Just1,2, Hanne Frenkel3, Rolf Gruetter3,4
1LIFMET, CIBM, EPFL, Lausanne, Switzerland; 2Department of Radiology, UNIL, Lausanne, Switzerland; 3LIFMET, EPFL, Lausanne, Switzerland; 4Department of Radiology, UNIL and HUG, Lausanne and Geneva, Switzerland
The present study examined the BOLD fMRI response of the rat barrel cortex upon sustained trigeminal nerve stimulation. Moreover, the alterations in the barrel cortex metabolite concentration due to prolonged functional activation were measured using 1H-MRS at 9.4T. The results demonstrate that sustained BOLD responses can be obtained reproducibly in the rat barrel cortex following prolonged trigeminal nerve stimulation. Furthermore, preliminary functional MRS (fMRS) results show changes in several metabolites in the barrel cortex and in particular an increase in lactate levels during barrel cortex activation.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1200_4123.pdf'))%7b%7d"  1200. The Utility of FMRI in Measuring Brain Plasticity Following Peripheral Nerve Injury
Christopher Paul Pawela1,2, Bharat B. Biswal3, Rupeng Li2, Anthony G. Hudetz4, Hani S. Matloub1, James S. Hyde2
1Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, WI, United States; 2Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, United States; 3Department of Radiology, University of Medicine and Dentistry of New Jersey, Newark, NJ, United States; 4Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United States
In this study brain reorganization following nerve injury and repair was followed for a twelve week period with BOLD fMRI in a rat model. Primary sensory functional return progressed over the entire study period whereas thalamic areas did not functionally return until the twelve week time point. This study demonstrates the utility of using BOLD fMRI as a substitute for conventional electrophysiology in studies of brain plasticity and has many applications outside of peripheral nerve injury and repair.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1201_753.pdf'))%7b%7d"  1201. Investigating the Role of Transcallosal Projections in Mediating Neuroplasticity Following Injury in a Rat Using FMRI
Jennifer I. Wood1,2, Suresh E. Joel1,3, Michael T. McMahon1,2, James J. Pekar1,2, Galit Pelled, 2,4
1F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States; 2The Russell H. Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States; 3The Russell H. Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States; 4F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute , Baltimore, MD, United States
Human and animal studies suggest the involvement of the transcallosal projection in shaping neuroplasticity following injury may be crucial in dictating the rehabilitation probability. This study was designed to investigate the role of the transcallosal pathways in mediating neuroplasticity following injury in a rat model of sensory deprivation. Using fMRI, we have studied the time course and the age-dependency of which the transcallosal projections effect cortical reorganization. The results demonstrate that the transcallosal projections involvement in neuroplasticity varies dependent on the age and the time following the injury and may introduce a critical consideration when choosing the right rehabilitation strategy.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1202_298.pdf'))%7b%7d"  1202. BOLD FMRI Assessment of the Functional Response to Taste Stimulation in Rat Brain
Ikuhiro Kida1, Yoko Hoshi1, Yoshinobu Iguchi1
1Integrated Neuroscience Research Team, Tokyo Institute of Pschiatry, Setagaya-ku, Tokyo, Japan
Some aspects of taste information processing have not been conclusively clarified, such as the process by which the gustatory cortex uses spatial codes to characterize taste information.We used BOLD fMRI measurements obtained at 7 T in an animal system to investigate the process by which taste information is encoded. Sucrose yielded a reproducible BOLD signal increase in the gustatory cortex. During taste stimulation, BOLD signals were also detected in the lip region of the primary somatosensory cortex, secondary somatosensory cortex, and amygdalae. This is the first study that used BOLD fMRI to observe gustatory activation in the rat brain.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1203_6942.pdf'))%7b%7d"  1203. Functional MRI of Cortico-Striato-Thalamal Circuit Using a Novel Flexible Polyimide-Based Microelectrode Array Implanted in Rodent Deep Brain
Pai-Feng Yang1, You-Yin Chen2, Jyh-Horng Chen1, Chen-Tung Yen3
1Electrical Engineering, National Taiwan University, Taipei, Taiwan; 2Electrical Engineering, National Chiao-Tung University, Hsinchu, Taiwan; 3Zoology, National Taiwan University, Taipei, Taiwan
This paper proposes a novel flexible MRI-compatible microelectrode array that leverages the stimulation and recording properties for neuroscience application. We perform functional MRI to investigate the cortico-striato-thalamal circuit with thalamic stimulation. Significant positive BOLD responses were observed in receptive field in upper lip region (S1ULp), barrel field (S1BF) and secondary somatosensory cortex (S2). Negative BOLD responses were revealed in caudate putamen (CPu). We inferred a limbic cortico-striatal loop might exist.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1204_5944.pdf'))%7b%7d"  1204. A Non Invasive Experimental Protocol for FMRI Studies: Investigation of the Basal Ganglia-Cortex Circuit in a Rat Model
Salem Boussida1, Amidou Traore2, Jean-Pierre Renou2, Franck Durif3
1INRA, UR370 QuaPA/NMR plateforme, Centre Clermont-Ferrand/Theix, F-63122 Saint Genès Champanelle, France; 2INRA, UR370 QuaPA/NMR plateforme, Centre Clermont-Ferrand/Theix, F-63122 Saint Genès Champanelle, France; 3CHU Clermont-Ferrand, Service de Neurologie, Clermont-Ferrand, F-63001, France.
Combination of blood– oxygen level-dependent functional magnetic resonance imaging (BOLD fMRI) and electrical hindpaw stimulation has been used as a standard model to study the somatosensory pathway and brain rehabilitation in rats. In the present study, we examined the feasibility of performing BOLD fMRI experiments on rat to investigate the activity of the basal ganglia (BG)-cortex circuit associated to hindpaw sensitive stimulation. These findings will have relevance in the fMRI studies dealing with physiopathology of neurodegenerative diseases such as Parkinson
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1205_5883.pdf'))%7b%7d"  1205. Refining the Sensory and Motor Ratunculus of the Rodent Upper Extremity: Evaluation of the C7 Nerve Root Using FMRI and Direct Nerve Stimulation
Patrick C. Hettinger1, Rupeng Li2, Ji-Geng Yan1, Hani S. Matloub1, Young R. Cho1, Matthew L. Runquist2, Christopher P. Pawela1, James S. Hyde2
1Plastic Surgery, Medical College of Wisconsin, Milwaukee, WI, United States; 2Biophysics, Medical College of Wisconsin, Milwaukee, WI, United States
The purpose of this study is to further define the ratunculus by observing cortical activity using BOLD fMRI during direct stimulation of the C7 nerve root. In this study, 7 Sprague-Dawley rats underwent implantable electrode placement on the C7 nerve root. BOLD response to nerve stimulation was then studied using a Bruker 9.4T MRI scanner. C7 nerve stimulation resulted in a small amount of activation in the S1FL region along with a large amount of activation within the M1/M2 regions. These findings are similar to the sensory and motor distributions described in human C7 nerve root literature.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1206_2689.pdf'))%7b%7d"  1206. Functional MRI Detects Chronically Enhanced Somatosensory Activation Maps Following Multiple Seizures in Rats.
Ursula I. Tuor1, Jennifer Vuong2, Jeffrey F. Dunn3, Tadeusz Foniok1, Dave Kirk4, Amy H. Henderson2, G Campbell Teskey2,5
1Institute for Biodiagnostics (West), National Research Council of Canada , Calgary, Alberta, Canada; 2Psychology, University of Calgary; 3Radiology, University of Calgary; 4Experimental Imaging Centre, University of Calgary; 5Anatomy and Cell Biology, University of Calgary, Calgary, Alberta, Canada
We used functional magnetic resonance imaging (fMRI) in rats to investigate whether following 20 repeatedly elicited seizures there are seizure-induced alterations in the somatosensory maps to forepaw stimulation. We observed increased areas of activation both acutely (1-3 days) and chronically (3-5 weeks) after experimental kindling induced epilepsy. The data indicate that there is considerable neuroplasticity and development of new pathways during the progression of epilepsy. Whether comparable plasticity of cortical maps is present in humans should be tested using fMRI in future studies, considering that such seizure-induced changes may be involved in producing interictal behavioural disturbances.

 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1207_5749.pdf'))%7b%7d"  1207. Layer-Specific FMRI of Photic Stimulation in the Rat Retina at 11.7 T
Yen-Yu Ian Shih1, Bryan H. De La Garza1, William J. Lavery1, Eric R. Muir1,2, Timothy Q. Duong1
1Research Imaging Institute, Ophthalmology/Radiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States; 2Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, United States
The retina is about 276 micron thick including the choroid and has highly organized laminar structures. This study reports, for the first time, the feasibility of high-resolution blood-volume fMRI to image layer specific (retinal and choroidal) visual responses in the rat retina at 11.7T up to 40x40x600 micron nominal resolution. Given that the choroid is behind the retina and the retinal pigment epithelium, it is generally inaccessible by optical techniques. Blood-volume fMRI thus could provide a unique means to evaluate lamina-specific functional changes in the rat retina where many retinal disease models are readily available.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1208_5188.pdf'))%7b%7d"  1208. Pass-Band Balanced Steady State Free Precession Functional MRI of the Mouse Retina
Eric Raymond Muir1,2, Sung-Hong Park3, Timothy Q. Duong2
1Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, United States; 2Research Imaging Institute, Ophthalmology/Radiology, UT Health Science Center San Antonio, San Antonio, TX, United States; 3Research Imaging Institute, Radiology, UT Health Science Center San Antonio, San Antonio, TX, United States
BOLD fMRI of the thin retina with the widely used EPI acquisition is challenging because the eye is a region of large magnetic inhomogeneity and high-resolution EPI is pushing the limits of gradient performance, resulting in susceptibility-induced signal drop out and image distortion. To overcome these limitations, we implemented a pass-band balanced steady state free precession (bSSFP) sequence for fMRI of the mouse retina at 45x45x500 µm. bSSFP has comparable temporal resolution and SNR per unit time as EPI, without the artifacts common in EPI. bSSFP fMRI could reliably detect layer-specific responses to hypoxic challenge in the mouse retina.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1209_3498.pdf'))%7b%7d"  1209. Simultaneous FMRI and Long-Term in Vivo Electrochemistry (LIVE): Identifying the Neurochemical Correlates of Functional Imaging Signals
John Lowry1, Karen Griffin2, Stephen McHugh3, Nicola Sibson4
1Department of Chemistry, National University of Ireland, Maynooth, Ireland; 2University College Dublin, Ireland; 3Department of Experimental Psychology, University of Oxford, Oxford, United Kingdom; 4Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford, Oxfordshire, United Kingdom
Long-term in-vivo electrochemistry (LIVE) enables real-time measurement of brain metabolites. Here we have simultaneously obtained BOLD fMRI and amperometric LIVE tissue oxygen data from rat cerebral cortex, during both increases and decreases in inspired oxygen. BOLD and tissue oxygen measurements demonstrated close correlation during both complete oxygen removal (negative responses) and increases in inspired oxygen (positive responses). Our findings demonstrate the feasibility of obtaining real-time metabolite information during fMRI acquisition. The results show that the BOLD signal provides a close correlate of the tissue oxygen dynamics or, alternatively, that tissue oxygen concentration can predict the magnitude of the BOLD response.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1210_5956.pdf'))%7b%7d"  1210. Evaluation of Cerebral Energy Demand During Graded Hypercapnia
Stefan Alexandru Carp1, Maria Angela Franceschini1, David Alan Boas1, Young Ro Kim1
1Martinos Center/Radiology, Massachusetts General Hospital, Charlestown, MA, United States
The cerebral metabolic rate of oxygen (CMRO2) is a physiological parameter closely linked to neural activation as well as to various disease states. Hypercapnic calibration is used to calibrate the BOLD-CBF-CBV relationship under the assumption of iso-metabolic blood flow increase during CO2 inhalation. Simultaneous near infrared optical measurements of cerebral blood volume, blood flow and oxygen extraction can also be used to monitor CMRO2 changes, albeit at low spatial resolution. We use these optical measurements during graded hypercapnia to test the iso-metabolic assumption, and demonstrate an apparent increase in brain metabolism at higher inhaled CO2 levels.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1211_5370.pdf'))%7b%7d"  1211. Dissociation of BOLD and Local Field Potentials
Wen-Ju Pan1, Matthew Magnuson1, Garth Thompson1, Waqas Majeed1, Dieter Jaeger2, Shella Keilholz1
1BME, Georgia Institute of Technology / Emory University, Atlanta, GA, United States; 2Biology, Emory University, Atlanta, GA, United States
To examine what extent consistence of the measurements between BOLD fMRI and local field potential (LFP), we evaluated BOLD and LFP simultaneously in rat somatosensory cortex with a combined measurement technique. The preliminary results indicated a dissociation between BOLD and LFP during low-level neural activity, which might mirror the limitation of neurovascular coupling, the bridge between BOLD and neural activity.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1212_7102.pdf'))%7b%7d"  1212. TR and TE Dependence on Low Frequency BOLD Fluctuations
Matthew Evan Magnuson1, Wenju Pan1, Waqas Majeed1, Garth Thompson1, Shella Keilholz1
1Biomedical Engineering, Georgia Institute of Technology / Emory University, Atlanta, GA, United States
Low freqnecy fluctuations in resting state BOLD data have often been used to map functional connectivity in the rat brain. These low frequency BOLD fluctuations contain contributions from CBF, CBV, and CMRO2. Each of these components have unique physiological time signatures and effective echo times; therefore, modification of the TR and TE used in resting state scans should result in altered contributions from each BOLD component. In this study we examine the dependence of the selection of the TR and TE variables on low frequency resting state data.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1213_754.pdf'))%7b%7d"  1213. Further Test and Validation of Saturation-Recovery T1 MRI Measurement for Imaging Absolute CBF Change
Xiao Wang1, Xiao-hong Zhu1, Yi Zhang1, Wei Chen1
1Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN, United States
The feasibility of saturation-recovery T1 MRI for imaging and quantifying absolute CBF change was further tested at 9.4T using a rat hypercapnia model on two aspects: using diffusion gradients to investigate macrovascular inflow contribution; and slab saturation with varied thickness to test the effects of blood transit distance on measuring CBF. The results indicate that the outcomes of CBF changes induced by hypercapnia were coincident with the literature reports, and not significantly affected by large-vessel inflow effect and blood transit time. The overall results demonstrate that the saturation-recovery T1 MRI is sensitive to microvascular perfusion; it provides a noninvasive and reliable imaging approach for studying cerebral perfusion changes induced by physiology or pathology perturbation.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1214_5176.pdf'))%7b%7d"  1214. In Vivo MR Measurement of Arterial Pulse Pressure in the Murine Aorta
Volker Herold1, Marco Parczyk1, Wolfgang Rudolf Bauer2, Eberhard Rommel1, Peter Michael Jakob1
1Department of Physics EP5, University of Wuerzburg, Wuerzburg, Bayern, Germany; 2Medizinische Universitätsklinik, University of Wuerzburg, Wuerzburg, Bayern, Germany
Mouse models are increasingly used to investigate functional and cardiovascular parameters. In this work we present an approach to noninvasively estimate the arterial pulse pressure by measuring the time dependant blood flow pulse and the local pulse wave velocity. By determining the complex impedance with solutions from the Navier-Stokes equations for incompressible fluids, the pressure pulse could be calculated from the accordant flow pulse. The present results are in good agreement with results from the literature obtained by invasive methods.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1215_4709.pdf'))%7b%7d"  1215. Problems for Motion Correction: Paradigm Correlated Motion Remains a Confounding Source for FMRI Artefacts.
Lubos Budinsky1, Benito de Celis Alonso2, Marina Sergejeva1, Andreas Hess2
1Institute of Pharmacology, FAU, Erlangen, Germany; 2Institute of Pharmacology, FAU, Erlangen, Germany
The undesired motion, which is correlated with the fMRI paradigm (often present in experiments which are using mechanical stimulation or painful stimulus) can create false areas of an activity, which could remain real BOLD activity areas and a signal time course. Here we present series of fMRI experiment with phantoms and dead animals to which the motion was induced by using an air driven device integrated into the animal cradle. Using results from these experiments we suggest strategy, how to deal with this problem even if conventional motion correction algorithm are not able to remove these false areas completely.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1216_7372.pdf'))%7b%7d"  1216. Functional MRI in the Rat at 9.4 T and 16.4 T
David Zsolt Balla1, Hannes M. Wiesner1, Gunamony Shajan1, Rolf Pohmann1
1High Field Magnetic Resonance Center, Max Planck Insitute for Biological Cybernetics, Tübingen, Baden-Württemberg, Germany
Functional MRI (fMRI) in animals at high magnetic fields keeps expanding our knowledge about the basics of neural processing but the specificity of the fMRI-signal is still under ongoing investigation. Yet, as the signal to noise ratio in MRI depends linearly on the magnetic field strength and calls for even stronger magnets for the detection of even smaller anatomical details, the relation between the functional MR-response and field strength can only be approximated with complex models. In this study the blood oxygenation dependent (BOLD) effect was measured and compared at 9.4 T and 16.4 T in the same animal with segmented gradient-echo (GE) and spin-echo (SE) echo planar imaging (EPI) sequence using optimal echo times for the respective field. Furthermore, high resolution fMRI acquisition at 16.4 T was performed up to a 50 µm in-plane accuracy and for an 8 s temporal resolution without the use of cryo-coils or coil-arrays.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1217_5156.pdf'))%7b%7d"  1217. BOLD, CBV, and CBF FMRI of Caudate Putamen in Rat Brain During Noxious Electrical Stimulation: Its Negative Hemodynamic Response to Neural Activities
Fuqiang Zhao1, Denise Welsh1, Mangay Williams1, Alexandre Coimbra1, Mark O. Urban2, Richard Hargreaves2, Jeffrey Evelhoch1, Donald S. Williams1
1Imaging Department, Merck Research Laboratories, West Point, PA, United States; 2Neuroscience Department, Merck Research Laboratories, West Point, PA, United States
In central nervous system, neuronal activity generally leads to increases in local venous blood oxygenation level (BOLD), cerebral blood flow (CBF), and cerebral blood volume (CBV). However, previous studies have reported that the neural activity in rat caudate putamen (CPu) during noxious electrical stimulation (NES) of paws causes BOLD and CBV decreases. To further understand the specific hemodynamic response in this anatomical structure and its temporal characteristics, BOLD, CBV, and CBF fMRI studies were performed in a rat brain slice containing the CPu. Our results suggest that the neural activity in the CPu during NES causes decreases in CBV and CBF, and an increase in CMRO2 which lasts >2 minutes after stopping the NES.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1218_1845.pdf'))%7b%7d"  1218. fMRI at 17.6 T and Optical Fiber-Based Ca2+-Imaging in Rodents
Albrecht Stroh1, Florian Schmid2, Afra Wohlschlaeger3, Valentin Riedl4, Jenny Kressel3, Cornelius Faber2
1Department of Neuroradiology, Technical University Munich, Munich, Germany; 2Institute for Clinical Radiology, University Hospital Münster, Münster, Germany; 3Department of Neuroradiology, Technical University Munich, Germany; 4Department of Neurology, Technical University Munich, Germany
n this study we tested the feasibility of rat fMRI at highest field strength in combination with an implanted optical fiber. We aim for the combination of fiber based optical Ca2+ imaging with functional magnetic resonance imaging (fMRI) at 17.6 T in vivo. We increased the temporal resolution of fMRI to 20 ms by applying k-space segmentation. Additionally, we conducted fiber-based Ca2+ imaging in mice upon electric forepaw stimulation, detecting neuronal population activity. Our study indicates that a multimodal approach combining a global method like fMRI with a spatially confined, highly specific method as optical Ca2+ imaging becomes amenable.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1219_1332.pdf'))%7b%7d"  1219. Layer Specific Detection of Inhibitory FMRI Response in Somatosensory Cortex Through Cortico-Cortical Interaction in Rats
Yoshiyuki Hirano1, Alan P. Koretsky2, Afonso C. Silva1
1CMU, LFMI, NINDS, NIH, Bethesda, MD, United States; 2FMMS, LFMI, NINDS, NIH, Bethesda, MD, United States
We investigated the laminar dependence of cortico-cortical interactions induced by paired bi-lateral somatosensory stimulation in ±-chloralose anesthetized rats. When compared to the response obtained at 0 ms ISI, the BOLD percent signal change in the S1FL responding to the late stimulus was decreased by 47 % at 40 ms ISI. The degree of suppression in layers III to V was stronger than suppression of fMRI changes in layers I-II and in bottom of layer VI. Our data shows that BOLD fMRI has sufficient spatial and temporal resolution to study cortical circuits, within functional columns and layers.
Animal fMRI: Pharmacologic Intervention
Hall B Thursday 13:30-15:30
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1220_6061.pdf'))%7b%7d"  1220. Anesthesia with Alpha-Chloralose in Rats: It Can Be Used for Longitudinal FMRI Studies
Benito de Celis Alonso1, Tanya Makarova1, Andreas Hess1
1Pharmacology and Toxicology, FAU Erlangen Nuremberg, Erlangen, Bayern, Germany
Animal experimentation in neurosciences requires the use of anesthetics for animal welfare and cooperation. Two of the most widely used anesthetics for functional magnetic resonance imaging (fMRI) of animals are Isofluorane (Iso) and Alpha-Chloralose (AC). Iso is an volatile drug shown to be suitable to obtain fMRI images at low concentrations(1). AC is an injectable anesthetic with strong functional-metabolic coupling but can create physiological problems. Therefore, it has been used as a non recoverable. There are studies were AC was used on human patients and others were AC was used to anaesthetize and recover dogs and cats (2,3). To our knowledge, the non-recoverable concept has not been challenged properly for fMRI. Here we present a protocol for AC anesthetic preparation and a fMRI study that shows that AC can be used as a recoverable anesthetic and has no effects on the fMRI results when animals are reused. Furthermore parallel behavioral studies on recovered rats show no effect on their brain and motor function.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1221_5251.pdf'))%7b%7d"  1221. Noradrenergic Modulation of Auditory Processing in the Songbird Brain.
Colline Poirier1, Tiny Boumans1, Michiel Vellema1, Geert De Groof1, Marleen Verhoye1, Jacques Balthazart2, Annemie Van der Linden1
1Bio-Imaging Lab, Antwerp, Belgium; 2GIGA Neurosciences, Liège, Belgium
Song learning in songbirds shares a large number of features with human speech acquisition. The songbird brain is thus an excellent model to study the neural bases of vocal learning and complex sound processing. The aim of this spin-echo BOLD fMRI study was to investigate the role of noradrenalin in conspecific songs and bird’s own song perception. Depletion of noradrenergic inputs resulted in an enhanced differential activation by socially relevant auditory stimuli in the secondary auditory regions of the songbird brain. These results suggest that noradrenalin might play an inhibitory role in song discrimination.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1222_6897.pdf'))%7b%7d"  1222. Role of Nitrite in Neurovascular Coupling: Nitric Oxide-Dependent and Independent Mechanisms
Barbora Piknova1, Ara Kocharyan2, Alan N. Schechter1, Afonso C. Silva2
1National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, United States; 2National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States
In addition to classic role of vasodilator, nitric oxide (NO) also acts as a neurotransmitter. NO role in neurovascular coupling and the possibility to restore the proper brain hemodynamics after its impairment in various NO-deficiency related diseases is of major importance. We studied the fMRI response to forepaw stimulation on á-chloralose anesthetized Sprague-Dawley rats at baseline, with NO production attenuated by nNOS inhibition and after NO and nitrite distribution. We found that exogenous NO and nitrite restore neurovascular response and that nitrite is more effective than direct NO donor. We hypothesize about additional vasodilatory pathways in case of nitrite metabolism.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1223_5434.pdf'))%7b%7d"  1223. Qualitative Differences in the Brain Activation Effects of Cocaine and Mdma Determined with Bold Fmri in Rhesus Monkeys
Kevin Sean Murnane1, Leonard Lee Howell1
1Neuroscience, Emory University, Atlanta, GA, United States
Both cocaine and methylenedioxymethamphetamine (MDMA) are drugs with a high propensity for abuse yet they have distinct behavioral and neurochemical effects. We compared the changes in blood oxygenation elicited by cocaine (0.3 mg/kg, i.v.) and MDMA (0.3 mg/kg, i.v.) in rhesus monkeys using BOLD fMRI. The effects of cocaine were localized to dorsal prefrontal cortex (PFC) whereas MDMA’s were localized to ventral PFC. Therefore, the neural circuitry engaged by these compounds appears to regulate their behavioral effects. As such, the use of fMRI allows for a novel approach to determine the mechanism of drugs of abuse.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1224_5198.pdf'))%7b%7d"  1224. De Novo Buprenorphine Phmri Effects in Conscious Rats Parallels Brain Activation in Humans
Lino Becerra1,2, Pei-Ching Chang1, James Bishop1, Jaymin Upadhyay1, Julie Anderson1, Gautam Pendse1, Smriti Iyengar3, Alexandre Coimbra4, Richard Baumgartner4, Adam Schwarz3, Jeffrey Evelhoch4, Erci Nisenbaum3, Brigitte Robertson5, Thomas Large5, David Bleakman3, Richard Hargreaves4, David Borsook1,2
1Imaging Consortium for Drug Development, McLean Hospital, Belmont, MA, United States; 2A A Martinos Center for Biomed. Imaging, MGH, Harvard Medical School, Charlestown, MA, United States; 3Eli Lilly and Co., Indianapolis, IN, United States; 4Merck and Co, West Point, PA, United States; 5Sepracor Inc., Marlborough, MA, United States
fMRI studies of rodents are confounded by the use of anesthetics, especially for the study of analgesics. Furthermore, there are no studies comparing pharmacological brain effects in humans and rodents of the same analgesics. In this work, we present results of pharmacological MRI (phMRI) of an opioid analgesic (buprenorphine) in conscious rats and compare the brain activations with results obtained in humans. Although brain structure and function differ between humans and rodents, some parallelism does exist and this thesis underpins much pre-clinical research. Translational results as presented here have the potential to bridge pre-clinical with clinical imaging studies.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1225_3447.pdf'))%7b%7d"  1225. A FMRI Study to Decipher the Regional Effects of an Intraperitoneal Glucose Dose in the Fasted Rat Model
Kim O'Toole1, Diana Cash1, Steve C R Williams1, Po-Wah So1
1Neuroimaging Department, Institute of Psychiatry, KCL, London, United Kingdom
Glucosensing neurones regulate membrane potential and firing rates in response to ambient glucose levels, and generally located in areas involved in neuroendocrine function, nutrient metabolism and energy homeostasis. Using BOLD-MRI, we have studied the effects of a single intraperitoneal glucose tolerance dose in the brain of a fasted rat model. Glucose induced BOLD-MRI signal increases in various regions of the brain, including the hypothalamus and hippocampus, which are known to contain glucosensing neurones. Thus, BOLD-MRI may be used to a non-invasive tool assess the functional role of nutrients in the brain under different physiological states.

 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1226_675.pdf'))%7b%7d"  1226. Effect of the Novel Anti-Depressant Agomelatine Determined by Pharmacological MRI in the Rat.
Karen Elizabeth Davies1, Inna V. Linnik1, Shane Mckie2, Jennifer A. Stark3, Simon Luckman3, Laure Sequin4, Elisabeth Mocaer4, Mark Millan4, Bill Deakin2, Steve R. Williams1
1Imaging Science & Biomedical Engineering, University of Manchester, Manchester, United Kingdom; 2Neuroscience and Psychiatry Unit, University of Manchester, Manchester, United Kingdom; 3Faculty of Life Science, University of Manchester, Manchester, United Kingdom; 4Institut de Recherches Internationales, Servier, Courbevoie, France
phMRI was used to determine brain areas activated by the novel anti-depressant agomelatine at 3 doses. T2*-weighted GE images were acquired continuously before and after injection of agomelatine or vehicle in isoflurane-anaesthetized rats. A pseudoblock analysis was performed in SPM5, revealing significant areas of activation and deactivation including cortical, hippocampal and caudate regions. There was a marked effect of dose with more brain areas, more total voxels and higher Z-scores at a dose of 20mg/kg compared to either 10 or 40mg/kg. Agomelatine acts at both melatonin and serotonin receptors and both receptors are likely to be involved in these responses.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1227_3364.pdf'))%7b%7d"  1227. Concurrent Pharmacological MRI and Electrophysiology to Investigate Neuropharmacological Modulation of Brain Function in the Rat
Christopher James Martin1, Nicola R. Sibson1
1Radiation Oncology and Biology, University of Oxford, Oxford, Oxfordshire, United Kingdom
The aim of this work was to combine pharmacological magnetic resonance imaging with electrophysiological recording of neuronal activity such that we might improve our understanding of: (1) the neural basis of neuroimaging signals; (2) the effects of neuropharmacological manipulations on neurovascular coupling and neuroimaging signals; and (3) neuroanatomical differences in the relationship between neuronal activity and neuroimaging signals. We report data from studies in which we use the serotonin (5-HT) releasing agent fenfluramine to increase endogenous 5HT levels and investigate the effects of this modulation on both baseline and stimulus-evoked fMRI signals and neuronal activity.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1228_3883.pdf'))%7b%7d"  1228. BOLD PhMRI in the Rat on a Clinical 3T Scanner Using Cocaine Challenge
Edwin Heijman1, Duncan Jack Hodkinson2, Roland van de Molengraaf3, Brian Henry4, Shane McKie5, Charles Sio1
1Philips Research Europe, Philips, Eindhoven, Netherlands; 2Imaging Science and Biomedical Engineering, The University of Manchester, Manchester, United Kingdom; 3Life Science Facilities, Philips Research, Philips, Eindhoven, Netherlands; 4Translational Medicine Research Centre, Schering-Plough, Singapore, Singapore; 5Neuroscience and Psychiatry Unit, The University of Manchester, Manchester, United Kingdom
In this study we investigated the potential applications of a clinical 3T system for pharmacological MRI (phMRI) in the rat brain. Using a human 3T MRI scanner, a dynamic SE-EPI BOLD sequence was implemented to determine alterations between pre- and post-injection of 5 mg/kg cocaine in male Sprague-Dawley rats under isoflurane anesthesia. Data analysis was performed using pseudoblock analyses. Cocaine-saline subtraction across the time series, showed significant activations in cortico-limibc areas of the motor, retrosplenial, and piriform cortex, extending to subcortical areas of the antero-dorsal hippocampus. We conclude that pre-clinical phMRI studies can be performed using 3T clinical scanners.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1229_3953.pdf'))%7b%7d"  1229. Effects of Doxorubicin on Brain Activity and Functional Connectivity in Rats
Alan S. Bloom1, Peter S. LaViolette2, Christopher R. Chitambar3, William Collier1, Sally J. Durgerian4, Balaraman Kalyanaraman2, Donna M. McAllister2, Carol L. Williams1, Kathleen M. Schmainda5
1Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, United States; 2Biophysics, Medical College of Wisconsin, Milwaukee, WI, United States; 3Neoplastic Diseases and Hematology, Medical College of Wisconsin, Milwaukee, WI, United States; 4Neurology, Medical College of Wisconsin, Milwaukee, WI, United States; 5Radiology and Biophysics, Medical College of Wisconsin, Milwaukee, WI, United States
The purpose of this research was to investigate “chemobrain” at a mechanistic level by determining the effects of doxorubicin, one of the agents commonly used for the adjuvant chemotherapy of breast cancer, on brain function using fMRI and fcMRI in a rat model. . We observed in a small group of rats treated weekly with DOX that it alters brain activation by sensory stimulation particularly in regions associated with vision and that we are able to demonstrate resting state functional connectivity MRI in the anesthetized rat and that it is decreased by DOX treatment, particularly in the visual system.
Athersclerosis & Coronary MRI
Hall B Monday 14:00-16:00
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1230_4471.pdf'))%7b%7d"  1230. Strong Collision Approximation to Predict Iron Volume Fraction in Ex Vivo Atherosclerotic Rabbit’s Aorta
Raimo P. Joensuu1, Louise M A Anderson, 12, Anna E. Larsson1, Li-Ming Gan1, Malin E. Palmér1, Paul D. Hockings1
1AstraZeneca R&D Molndal, Mölndal, Sweden; 2Chalmers University of Technology, Gothenburg, Sweden
The purpose of this work was to evaluate the suitability of the strong collision approximation to predict the USPIO volume fraction in atherosclerotic rabbits’ vessel wall from the transverse relaxation time, T2*. There was an excellent agreement (R2 = 0.98) between the theory and the measurements for volume fractions larger than 15 ppm. For lower volume fractions the theory agrees with the measurements poorly. The strong collision model may predict correctly the volume fraction from the T2* map when every voxel contains a relatively high number of magnetic particles but fails if the region contains also voxels with few or no particles.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1231_2083.pdf'))%7b%7d"  1231. Heparin-Polynitroxide Derivatives: First Application as Site Specific MRT Imaging Contrast Media for Vascular Wall
Maxim V. Terekhov1, Vasily Sen'2, Valery Golubev2, Stefan Weber3, Alexander W. Scholz4, Thomas Muenzel5, Andrei L. Kleschyov5, Laura Maria Schreiber3
1Department of Radiology, Section of Medical Physics, Johannes Gutenberg University Medical Center Mainz, Mainz, Germany; 2Institute of Problems of Chemical Physics, Russian Academy of Sciences, Russian Federation; 3Department of Radiology, Section of Medical Physics, Johannes Gutenberg University Medical Center Mainz, Mainz, Germany; 4Department of Anesthesiology, Johannes Gutenberg University Medical Center Mainz, Mainz, Germany; 5II-Department of Medicine, Johannes Gutenberg University Medical Center Mainz, Mainz, Germany
Cyclic nitroxides e.g. TEMPO are stable free radicals with multiple applications in MRI. Heparin is known to have a high affinity for the vascular extracellular structures. We propose that TEMPO could be delivered to the vascular wall by means of heparin-polynitroxide (HNR) derivatives where the nitroxide is linked with the heparin macromolecule. The T1,2 measurements show that HNR complexes provide the relaxivity contrast comparable with Gd-based media. The pilot MRI experiment with ex-vivo labeling of vascular wall with HNR demonstrate high potential of functionalized polynitroxide targeted to the specific structures of the vascular wall for diagnostic and therapeutic purposes.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1232_22.pdf'))%7b%7d"  1232. Clinical 3.0T Magnetic Resonance Scanner Can Be Used for Imaging of Mouse Atherosclerotic Lesions¦
Xubin Li1,2, Huidong Gu1, Hongqing Feng1, Xiangke Du2, Bensheng Qiu1, Xiaoming Yang1
1Image-Guided Bio-Molecular Intervention Researchers, Department of Radiology; Institute for Stem Cel, University of Washington School of Medicine, Seattle, WA, United States; 2Radiology, Peking University People's Hospital, Beijing, China
The aim of this study was to explore the possibility of generating high-resolution MR images of atherosclerotic aortic walls/plaques of mice using a clinical 3.0 Tesla MR scanner. This study demonstrates that clinical 3.0T MR scanners can be used for high-resolution imaging of atherosclerotic vascular walls and lesions in mice, which is guaranteed with a specific mouse RF coil, an effective ECG-gating system, and a BB-MRI sequence.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1233_1832.pdf'))%7b%7d"  1233. Detection of Coronary Artery Wall Inflammation in a Porcine Model Using Non-Contrast Enhanced MRI
Steen Fjord Pedersen1, William P. Paaske2, Troels Thiem3, Samuel A Thrysøe, Erling Falk3, Steffen Ringgaard, Won Yong Kim4
1Dept. of Cardiology, and MR-center, Aarhus University Hospital, Skejby, Aarhus, Denmark; 2Dept. of Cardiothoracic and Vascular Surgery T, Aarhus University Hospital, Skejby; 3Dept. of Cardiology, Aarhus University Hospital, Skejby; 4dept. of Cardiology, and MR-center, Aarhus University Hospital, Skejby
Inflammation seems to play a key role in destabilization of atherosclerotic plaques. Detection of Inflammatory activity within atherosclerotic plaques therefore has the potential to distinguish between vulnerable and stable plaques. Using a balloon injured porcine coronary artery, we examined whether edema as a sign of inflammation could be detected in the vessel wall by MRI using a T2-STIR (known to detect edema). After injury, the T2-STIR images showed a significant increase in vessel wall enhancement of 143% (CI95 = [39.6 - 142.5]; and areas with signal enhancement correlated well to inflammation and edema confirmed by histopathology.

 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1234_4263.pdf'))%7b%7d"  1234. Coronary Artery Plaque Imaging: Comparison of Black-Blood MRI and 64-MDCT
Yi He1, Zhaoqi Zhang1, Qinyi Dai1, Wei Yu1, Biao Lu1, Zhanming Fan1, Jing An2, Lixin Jin3, Guobin Li4, Wolfgang Rehwald5, Renate Jerecic3, Debiao Li6
1department of radiology, An zhen hospital, Beijing, China; 2Siemens Mindit Magnetic Resonance, Siemens Healthcare, MR Collaboration NE Asia; 3Siemens Limited China, Siemens Healthcare, MR Collaboration NE Asia; 4Siemens Mindit Magnetic Resonance Ltd; 5Siemens Healthcare USA; 6Northwestern University, Chicago USA
This study was to evaluate the ability of black-blood coronary wall MRI to identify and classify coronary plaques by comparing with 64-MDCT.15 patients underwent black-blood coronary wall MRI and coronary 64-MDCT. In MRI, the plaque burden, maximal wall thickness, SNR, CNR in the coronary walls containing plaques were greater than those of the normal coronary walls.The SNR in the soft plaque was greater than those in calcified and mixed plaques. The conclusion was coronary wall MRI can identify coronary plaques, and has the potential to differentiate plaque types based on signal intensity.

 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1235_424.pdf'))%7b%7d"  1235. Non-Contrast Coronary Vessel Wall and Plaque Imaging Using Inversion Recovery Prepared Steady State Free Precession: Comparison with Plaque Characterization of 64 Detector Row CT
Takeshi Ishimoto1, Yasuyo Taniguchi2, Tosiaki Miyati3, Momoe Kawakami4, Takayuki Ikeda5, Hisaya Kusabe5
1Division of Health Science, , Graduate school of Medical, Kanazawa University, Kanazawa, Ishikawa, Japan; 2Department of Cardiology, Hyogo Brain and Heart Center, Himeji, Hyogo, Japan; 3Division of Health Science,, Graduate school of Medical, Kanazawa University, Kanazawa, Ishikawa, Japan; 4Radiology and Clinical Laboratory, Hyogo Brain and Heart Center, Himeji , Hyogo, Japan; 5Radiology and Clinical Laboratory, Hyogo Brain and Heart Center, Himeji, Hyogo, Japan
In the study, we sought to determine whether coronary vessel wall imaging using inversion recovery prepared SSFP correlate with atherosclerosis detected by 64 raw MDCT. IR-SSFP can be used to non-invasively visualize the coronary vessel wall and to detect the presence of (sub)clinical coronary atherosclerosis . HSI of IR-SSFP may be indicative of plaque inflammation and/or hemorrhage. Therefore, coronary plaque vulnerability could be predicted by cardiac MRI. Further studies are needed to define the importance of these findings in the detection and treatment of vulnerable plaques.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1236_5247.pdf'))%7b%7d"  1236. Contrast-Enhanced Whole Heart Coronary MRI with Bolus Infusion of Gadobenate Dimeglumine at 1.5T
Peng Hu1, Jonathan Chan, Jouke Smink2, Beth Goddu, Kraig V. Kissinger, Lois A. Goepfert, Thomas H. Hauser, Neil M. Rofsky3, Warren J. Manning, Reza Nezafat
1Beth Israel Deaconess Medical Center, Boston, MA, United States; 2Philips Healthcare; 3Radiology, Beth Israel Deaconess Medical Center
We sought to investigate the contrast injection timing and rate for contrast-enhanced coronary artery MRI and compared the images acquired with optimized contrast timing to non-contrast T2-prep whole-heart SSFP coronary MRI at 1.5T. We studied time-resolved blood T1 after gadobenate dimeglumine (Gd-BOPTA) injection using three infusion schemes (bolus, slow infusion and hybrid). Subsequently, we evaluated an isotropic contrast-enhanced whole-heart coronary MRI method at 1.5T using an inversion-recovery SSFP sequence acquired after a bolus infusion of Gd-BOPTA. The contrast-enhanced coronary MRI increased blood SNR by 36% and increased coronary-myocardium CNR by 101%. There was no significant difference in image quality.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1237_4006.pdf'))%7b%7d"  1237. 3D Flow-Insensitve Coronary Vessel Wall Imaging Using Phase Sensitive Inversion Recovery
Jingsi Xie1, Himanshu Bhat1, Zhaoyang Fan1, Debiao Li1
1Departments of Radiology and Biomedical Engineering, Northwestern University, Chicago, IL, United States
Developed a 3D flow-insensitive coronary vessel wall imaging technique.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1238_373.pdf'))%7b%7d"  1238. Contrast-Enhanced Whole-Heart Coronary MRA at 3.0T for the Evaluation of Cardiac Venous Anatomy
Heng Ma1, Lan Ge2, Qi Yang1, Dong Xu1, Han Li1, Qing Tang1, Jiangtao Liu1, Wen Qin1, Jing An3, Lixin Jin4, Renate Jerecic4, Xiangying Du1, Kuncheng Li1, Debiao Li2
1Xuanwu Hospital, Capital Medical University, Beijing, China; 2Radiology, Northwestern University, Chicago, IL, United States; 3Siemens Mindit Magnetic Resonance Ltd, Shenzhen, China; 4Siemens Ltd, Healthcare Sector, China
Fifty-one subjects underwent contrast-enhanced whole-heart coronary magnetic resonance angiography at 3.0T. All major cardiac veins, except for the vein of Marshall, could be depicted successfully.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1239_755.pdf'))%7b%7d"  1239. A New Approach for Single Breath-Hold Whole Heart Coronary MRA Using Highly-Accelerated Parallel Imaging with a 32-Element Coil Array
Jian Xu1,2, Daniel Kim3, Ricardo Otazo3, Sven Zuehlsdorff4, Xiaoming Bi4, Bernd Stoeckel1, Daniel Sodickson3
1Siemens Medical Solutions USA Inc., New York, NY, United States; 2PolyTechnic Institute of NYU, New York, NY, United States; 3Center for Biomedical Imaging,Department of Radiology, New York University, New York, NY, United States; 4Siemens Medical Solutions USA Inc., Chicago, NY, United States
To develop a new approach for high resolution 3D whole heart coronary MRA in a single breath-hold, which offers the potential to enhance imaging efficiency and spatial resolution without apparent misregistration between external RF coil calibration scan and imaging scan.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1240_3193.pdf'))%7b%7d"  1240. Whole-Heart Water/Fat Resolved Spiral Imaging for Coronary MRA and Fatty Myocardial Infiltrations
Peter Börnert1, Kay Nehrke1, Holger Eggers1, Peter Koken1
1Philips Research Europe, Hamburg, Germany
Fat suppression is essential to improve contrast in MR coronary angiography (CMRA) but fat also contains helpful diagnostic information. In particular, the intra-myocardial fat represents an important diagnostic indicator that could have high prognostic value. Therefore, in this work whole heart CMRA-type imaging is proposed that delivers both, the coronary tree and the fat signal distribution at the same spatial resolution. Dixon-based chemical shift encoded spiral imaging is used allowing to separate water and fat and to benefits from the ƒ´Bo -based off-resonance correction. This concept was applied and validated in volunteers and shows that efficient CMRA and intra-myocardial fat detection is possible with large volume coverage.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1241_4408.pdf'))%7b%7d"  1241. MR-Imaging of the Coronary Arteries of Mice in Vivo
Arno Nauerth1, Erich Treiber1, Claudia Oerther1, Ulrich Flögel2
1Bruker BioSpin MRI GmbH, Ettlingen, Germany; 2Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany
In this study the dynamic filling of the coronary arteries in mice was visualized by using a cryogenically cooled surface coil for SNR improvement and by using a self-gated method with full heart coverage which corrects for cardiac and respiratory motions. The results presents volume-targeted cine-MIPs.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1242_710.pdf'))%7b%7d"  1242. Analysis of Small Dilation Detection in Coronary Angiography
Travis B. Smith1, Krishna S. Nayak1
1Electrical Engineering, University of Southern California, Los Angeles, CA, United States
Estimation of coronary artery cross-sectional lumen area from MR images is now being used to assess the relationship between atherosclerosis and vasomotion response to endothelial-dependent stimuli. Typically, cross-sectional images of the coronary lumen acquired before and after administration of such stimuli are fit to circular templates to estimate the change in area. These studies have reported very small mean changes in lumen area. In this work, we develop a statistical framework for the detection of these subtle changes in lumen area using two images. We use this framework to relate detection performance to SNR requirements and minimum detectable dilation.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1243_5349.pdf'))%7b%7d"  1243. Analysis of Coronary Vein Motion: Implications for MR Coronary Vein Venography
Pierre John Watson1, Jonathan D. Suever2, John N. Oshinski1
1Radiology, Emory University, Atlanta, GA, United States; 2Biomedical Engineering, Georgia Institute of Technology
The periods of low motion of the coronary veins during the cardiac cycle were quantified using 3D magnetic resonance coronary venograms (cMRV) in 16 patients with ischemic heart disease. The temporal location and duration of these periods were determined using the frame-to-frame displacement method on the coronary sinus. The patients were classified as either systolic or diastolic dominant based on the duration of the periods. The majority of patients were systolic dominant although about a third of the patients either had a very short diastolic period or lacked it completely.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1244_4619.pdf'))%7b%7d"  1244. Motion Correction Coeeficient Pre-Analysys Method for Whole-Heart Magnetic Resonance Coronary Angiography (Wh Mrca) for Use in a Clinical Setting
Yuki Ohmoto1, Rieko Ishimura2, Takashi Yoshida3, Miho Yabuyamada3, Junji Takahashi3, Shigehide Kuhara4, Sachiko Isono4, Ayako Ninomiya4, Mitsue Miyazaki4, Hiroyuki Tsuji1, Yasuji Arase1
1Health Management Center, Toranomon Hospital, Minato-ku, Tokyo, Japan; 2Cardiovascular Center, Toranomon Hospital; 3Radiology, Toranomon Hospital; 4MRI systems Division, Toshiba Medical Systems Corporation
Whole-Heart Magnetic Resonance Coronary Angiography (WH MRCA) is a very useful tool for screening for coronary artery disease and usually performed during free breathing with real time motion correction (RMC). We have developed a Motion Correction Coefficient Pre-Analysis Method to obtain an appropriate RMC coefficient before the WH MRCA scan and performed the feasibility study of this method by evaluating the relation between the coefficient of RMC and the image quality for health screening cases retrospectively. The results showed that this method is expected to be very useful in the clinical application of WH MRCA.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1245_2907.pdf'))%7b%7d"  1245. Global-To-Local Tandem Method for Detecting the Coronary Stationary Period for Whole-Heart Magnetic Resonance Coronary Angiography (Wh Mrca)
Ayako Ninomiya1, Shigehide Kuhara1, Tomohisa Okada2, Tetsuo Sato3, Kotaro Minato3, Shoutaro Kanao2, Kaori Togashi2
1MRI Systems Division, Toshiba Medical Systems Corporation, Otawara-shi, Tochigi, Japan; 2Kyoto University Hospital, Kyoto, Japan; 3Nara Institute of Science and Technology, Nara, Japan
We have developed the Global-to-Local Tandem Method to detect the coronary stationary period for WH MRCA, in which the coronary stationary period is roughly analyzed by the global method and then more precisely analyzed by the local method. We have also conducted clinical feasibility studies. Results suggest that The Global-to-Local Tandem Method can robustly detect the coronary artery stationary period in WH MRCA (Whole Heart Magnetic Resonance Coronary Angiography). It is therefore concluded that this method should prove to be very useful for clinical WH MRCA examinations.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1246_7153.pdf'))%7b%7d"  1246. Retrospective Motion-Adapted Smart Averaging for Free-Breathing Cardiac MRI
Alan Christopher O'Connor1,2, Mehdi Hedjazi Moghari1, Peng Hu1, Dana C. Peters1, Warren J. Manning1, Reza Nezafat1, Roger Ware Brockett2
1Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States; 2SEAS, Harvard University, Cambridge, MA, United States
Navigator gating is an accepted method for reducing the effect of respiratory motion in cardiac MRI; however, it does not exploit the fact that different spatial frequencies are more or less sensitive to the respiratory motion. We present a smart motion-adapted averaging method that retrospectively corrects for respiratory motion by combining data from k-space lines of multiple acquisitions using weights determined by the navigator signal.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1247_6246.pdf'))%7b%7d"  1247. CoSMo: Compressed Sensing Motion Correction for Coronary MRI
Mehdi Hedjazi Moghari *1, Mehmet Akçakaya *,12, Alan O'Connor, 12, Peng Hu1, Vahid Tarokh2, Warren J. Manning1, Reza Nezafat1
1Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States; 2Harvard University, Cambridge, MA, United States
We examine the feasibility of using compressed sensing to reduce artifacts due to respiratory motion. Respiratory motion causes image artifacts and ghosting in cardiac imaging. Respiratory navigators are one of the methods used to mitigate these artifacts for free-breathing scans, where k-space lines falling outside a pre-defined gating window are reacquired until the whole k-space is filled. In this study, we introduce CoSMo, a compressed sensing-based method for reconstructing images without having to reacquire k-space lines rejected by the navigator.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1248_2819.pdf'))%7b%7d"  1248. New Approach for Patient-Specific Estimation of Cardiac Motion Due to Respiration
Florencio Rusty Baldomaro Punzalan1, Tetsuo Sato1, Tomohisa Okada2, Shigehide Kuhara3, Kaori Togashi2, Kotaro Minato1
1Graduate School of Information Science, Nara Institute of Science and Technology, Ikoma, Nara, Japan; 2Department of Diagnostic Radiology, Kyoto University Hospital, Kyoto, Japan; 3MRI Systems Division, Toshiba Medical Systems, Tochigi, Japan
Respiratory motion compensation for cardiac imaging requires a patient-specific knowledge of the heart’s motion. In this study, the respiratory-induced motion of the upper and lower part of the heart was investigated during free-breathing. We observed significant inter-subject variation of upper and lower heart motion tracking factors. The tracking factor for the lower part is larger than the upper part for all subjects. This will help motion models account for the different displacements and velocities of coronary vessels located in the atrio-ventricular groove (RCA and LCX) and the apex (LAD) which are located in the lower and upper heart, respectively.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1249_271.pdf'))%7b%7d"  1249. Which Factors Influence MRI In-Stent Lumenvisibility of Coronary In-Stent Stenosis? an In-Vitro Model Investigation
Gerrit Schönwald1, Gregor Schaefers2, Georg Haltern3, Brigitte Kipfmüller4
1University Witten/Herdecke, Gelsenkirchen, NRW, Germany; 2MR:comp GmbH, Gelsenkirchen, Germany; 3HELIOS Heart Center , Wuppertal, Germany; 4University of applied Science Gelsenkirchen, Germany
Purpose Development of a standardized procedure to evaluate which factors need to be optimized for quantification of in-stent stenosis by analysis of lumenvisibility in a static MRI model of coronary in-stent stenosis. Methods Stents were placed into tubes and equipped with artificial restenosis made of a pre-investigated material. The tubes were placed in a phantom. A 1.5 and a 3 Tesla MR-system were used. Results Quantification of in-stent stenosis was limited in stainless steel stents and cobalt-chromium stents by artifacts. Tantalum stent showed a lower rate of artifacts. Conclusion Image quality was strongly depended from stent material and stent design.
Vessel Wall Imaging (Non-Coronary)
Hall B Tuesday 13:30-15:30
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1250_6003.pdf'))%7b%7d"  1250. Comparison Between Ungated Multi-Slice and Gated Single-Slice Double Inversion Recovery Prepared Black-Blood Fast Spin Echo Sequences Applied at 3T
Andrew J. Patterson1, Victoria E. Young1, Martin J. Graves1, Jonathan H. Gillard1
1Radiology, University of Cambridge, Cambridge, England, United Kingdom
Double inversion recovery prepared, fast spin echo, black blood imaging has been widely used for assessing carotid plaque tissue composition. The protocol is used to obtain T1-, T2- and PD-weighted contrast. This study compares the between subject and within subject differences in the signal-to-noise ratio and the contrast-to-noise ratio from single- and multi-slice acquisition using both a gated and ungated trigger.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1251_5045.pdf'))%7b%7d"  1251. Black Blood Imaging of Carotid Plaque Using Spatial Labeling with Multiple Inversion Pulses Prepared Spoiled Gradient Recalled Sequence
Hao Shen1, Guang Cao2, QingJun Wang3
1Applied Science Laboratory, GE Healthcare, Beijing, China; 2Applied Science Laboratory, GE Healthcare, Hong Kong, China; 3Department of Radiology, Chinese PLA General Hospital, Beijing, China
Black blood is important in carotid plaque characterization. In this study, we developed a black blood spoiled gradient recalled sequence by using spacial labeling with multiple inversion pulses preparation.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1252_4085.pdf'))%7b%7d"  1252. High Resolution 3D Black Blood Carotid Artery Imaging Using 3D TSE Sequence with Non-Selective Refocusing RF and Inner Volume Imaging Technique.
Seong-Eun Kim1,2, Scott McNally2, Laura K. Findeiss2, Jordan Hulet3, John Roberts1,2, Eun-Kee Jeong1,2, Dennis L. Parker1,2, Gerald S. Treiman4,5
1UCAIR, University of Utah, Salt Lake City, UT, United States; 2Department of Radiology, University of Utah, Salt Lake City, UT, United States; 3Department of Biomedical Informatics, University of Utah; 4Department of Surgery, University of Utah, Salt Lake City, UT, United States; 5Veterans Affair, VASLCHCS, Salt Lake City, UT, United States
2D TSE with DIR is the current technique for identification of the component of carotid plaque. This approach is limited by inadequate spatial resolution that is often necessary to identify small areas of plaque components. 3D imaging offers the potential to improve spatial resolution. We have utilized 3D TSE with non-selective 180o RF and have implemented an inner volume imaging(IVI) technique. The non-selective 180o RF allow significantly more echoes to be acquired resulting in more efficient 3D scan. IVI technique reduces the field of view in the phase encoding direction and requires fewer phase encoding line, further reducing scan time.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1253_251.pdf'))%7b%7d"  1253. Intravascular 3.0T MR Imaging: A Feasibility Study in Swine
Yanfeng Meng1,2, Feng Zhang1, Huidong Gu1, Jinnan Wang3, Chun Yuan1, Zhaoqi Zhang2, Bensheng Qiu1, Xiaoming Yang1
1Radiology, University of Washington, Seattle, WA, United States; 2Radiology, Beijing Anzhen Hospital, Beijing, China; 3Clinical Sites Research Program, Philips Research North America, Briarcliff Manor, NY, United States
This study was to validate the feasibility of generating intravascular 3T MRI of deep-seated arteries of near-human-sized swine by using a 3T-MR compatible MR-imaging guidewire (MRIG). For in vitro testing, we compared SNRs generated by a 0.032-inch MRIG and surface coils. For in vivo validation, we performed intravenous MRI of the parallel-run iliofemoral arteries with this MRIG. The SNR by the MRIG was higher than surface coils, and the iliofemoral arterial walls were clearly delineated with the MRIG at a higher SNR than surface coils. This study establishes the groundwork for further intravascular 3T MRI of deep-seated arteries in humans.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1254_1982.pdf'))%7b%7d"  1254. Histology Assisted Validation of Automatic Detection of Soft Plaque in Vessel Wall Images by Using Optimal Number of MR Sequences
Ronald van 't Klooster1, Andrew J. Patterson2, Victoria E. Young2, Jonathan H. Gillard2, Johan H.C. Reiber1, Rob J. van der Geest1
1Division of Image Processing, Department of Radiology, Leiden University Medical Center, Leiden, Netherlands; 2University Department of Radiology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom
Extensive MR vessel wall imaging protocols are used to identify unstable plaques, which play an important role in the progression of atherosclerosis. Comparison was made between automatic plaque detection, by a supervised classification system, and histology assisted manual segmentation. Experiments show that the automatic detection of unstable plaque is in good agreement with the manual segmentation. Moreover, the STIR and DWI sequences show an improvement over the T2w and PDw sequences. Automatic detection of soft plaque may be feasible by using a limited number of MR sequences, saving both MRI system and image analysis time.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1255_2193.pdf'))%7b%7d"  1255. Improved Calcification Detection Accuracy on Human Atherosclerotic Plaque Using Ultra-Short TE (UTE) Imaging
Jinnan Wang1, Marina S. Ferguson2, Niranjan Balu2, Chun Yuan2, Peter Boernert3
1Clinical Sites Research Program, Philips Research North America, Seattle, WA, United States; 2University of Washington; 3Philips Research Europe
Ultra-short TE (UTE) images can provide positive contrast for short T2 species when combined with imaging techniques like dual-echo subtraction or magnetization preparation. Although the calcified regions identified on UTE images were demonstrated to agree with CT images, its accuracy has not been validated against histology. This study is aimed at comparing the accuracy of UTE calcification detection in human carotid plaques against regular turbo spin echo MR images, as well as validating it against histology.
 HYPERLINK "javascript:if(top.frames[gi_SearchJSFrameNum].uf_LoadPDF('../files/1256_5990.pdf'))%7b%7d"  1256. Response of the Carotid Vessel Wall to Lipid-Lowering Therapy: Time Course of T1 and T2-Weighted Signal Variation
Li Dong1, William Sean Kerwin1, Chun Yuan1, Xue-Qiao Zhao1
1University of Washington, Seattle, WA, United States
We hypothesized that lipid-lowering therapy leads to biological changes in arterial walls that result in altered MRI contrast, even in the absence of a developed necrotic core. In a carotid MRI study of atorvastatin, 42 subjects exhibited no necrotic core. Within this group, no measurable change in wall thickness occurred over 3 years, but a highly significant (py£y¤y¥y¨yªyzzz'z(z6z7zEzFzTzUzczdzrzsz„z…z•z–z—zëÞÎÃ榶—Ί{j{j{j{j{j{j{j{jV'hàe¹h§Y›0J5B*\mHphsH hàe¹h§Y›B*H*mHphsHhàe¹h§Y›B*mHphsHhàe¹h§Y›0JB*phhàe¹h§Y›0J5B*phhàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phhàe¹h§Y›B*phjhàe¹h§Y›B*Uphhàe¹h§Y›B*CJph'hàe¹h§Y›0J5B*\mHphsH—z˜zîzïz@{A{±{²{ú{û{W|Z|Ò|Ô|Ö~×~Ø~>?@CEžŸ ±óèóèóèóèØ˼˭˛‹è‹~n~_‹RCh!‹h§Y›B*mH phsH hàe¹h§Y›0JB*phhàe¹h§Y›0J5B*phhàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*phhàe¹h§Y›B*H*ph±´ÁÂÐÓáâòó€€€€_€`€°€±€ï€ð€STƒ‘ƒ“ƒ™ƒšƒ›ƒ„„„ïàïàïàïàïàïÌ¿´¿´¿´¿´¤—ˆ—ˆ—vf´fYhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*phhàe¹h§Y›B*H*ph'h!‹h§Y›0J5B*\mH phsH h!‹h§Y›B*mH phsH  h!‹h§Y›B*H*mH phsH „„„p„q„r„ƒ„„„”„•„¨„©„½„¾„¿„À„ï„ð„'…(…\‡^‡y‡ˆ‰‰‰à‰â‰ä‰ê‰î‰ïâÓö«ž«ž«ž«žŽž«ž«ŽrcQëÃâïâ#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*H*phhàe¹h§Y›B*phhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uphhàe¹h§Y›0J5B*phhàe¹h§Y›0JB*phhàe¹h§Y›0J56B*phî‰@ŠAŠBŠUŠVŠgŠhŠ~ŠŠ‘Š’Š¤Š¥Š´ŠµŠ¶Š·Š ‹ ‹a‹b‹ŽŽŽmŽnŽoŽñáÔÅ´Å´Å´Å´Å´Å´ “ˆ“ˆxkYáˆáLhàe¹h§Y›0JB*ph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*phhàe¹h§Y›B*H*ph'h!‹h§Y›0J5B*\mH phsH  h!‹h§Y›B*H*mH phsH h!‹h§Y›B*mH phsH hàe¹h§Y›0JB*phjhàe¹h§Y›B*Uphhàe¹h§Y›0J5B*phoŽrŽtŽãŽäŽåŽôŽõŽ$%78FGVWXYª«øùKLjkÿ’““f“g“h“k“m“Ǔȓɓߓâ“ð“ïâÓö«ž«ž«ž«ž«ž«ž«žŽž«ž«ž«ž«ŽoëÃâïâÓö«ž«#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*H*phhàe¹h§Y›B*phhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uphhàe¹h§Y›0J5B*phhàe¹h§Y›0JB*phhàe¹h§Y›0J56B*ph+ð“ó“””””(”+”*B*phhàe¹h§Y›0J5B*H*phhàe¹h§Y›0J5B*phhàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phhàe¹h§Y›B*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJphll l!l1l2lFlGlXlYlnlolplqlŒll¾l¿lílîlïlVnWnEoFoGo­o®o¯o²o´oóèóèóèóèóèóØǸǸǸ¤•ˆ•ˆØxèxk[khàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uphhàe¹h§Y›B*CJphhàe¹h§Y›B*CJH*ph'hàe¹h§Y›0J5B*\mH phsH hàe¹h§Y›B*mH phsH  hàe¹h§Y›B*H*mH phsH hàe¹h§Y›0J5B*\phhàe¹h§Y›B*phhàe¹h§Y›B*H*ph´o2p3p4pNpOp`paplpmpnpopôpõpkqlqíqîq&t'totptqt×tØtÙtÜtÞtJuKuLuUuVu`uaubucu£u¤uñáÔɼɼɼ¬¼É¼É¼É¬ŸŸ~áÉáqaqñáÔɼɼ¬¼É¬hàe¹h§Y›0J56B*phhàe¹h§Y›0JB*ph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*H*phhàe¹h§Y›B*phhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uphhàe¹h§Y›0J5B*ph&¤u±x²x³xyyyy yQyRySy_y`yqyryy‚yƒy„yãyäy{|||}|ã|ä|å|è|ê|A}B}C}M}N}Z}[}g}h}w}x}y}z}~òàÐÅи¨¸™ÐŒÅÅÅoÅoòàÐÅи¨¸™ÐŒÅÅÅÅoÅhàe¹h§Y›0J5B*\phhàe¹h§Y›B*H*phhàe¹h§Y›0JB*phhàe¹h§Y›0J5B*phhàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phhàe¹h§Y›B*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJph+||C}y}~~€рî€с‚ë‚’†W‡«‡úˆƒ‹gŒ´Œ ’½’ñãÕǹ«ñãÕÇñãÕÇñãÕÇñgd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.~~}~~~Á€€î€ï€UVWZ\ρЁсځہìí‚‚‚‚‚‚p‚q‚ê‚ë‚‘†’†“†ù†ú†û†þ†‡U‡V‡W‡óèØ˹è©è©œŒœ}©pèóèóèóèóØóèóèØ˹©è©œŒœ}©phàe¹h§Y›0JB*phhàe¹h§Y›0J5B*phhàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*phhàe¹h§Y›B*H*ph(W‡d‡e‡r‡s‡‚‡ƒ‡•‡–‡©‡ª‡«‡¬‡ˆˆˆˆùˆúˆ¸‰¿‰áŠèŠ‚‹ƒ‹„‹ê‹ë‹ì‹ðßðßðßðßðß˾³¾³¾³£–‡–‡–ue³eXhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›6B*CJphhàe¹h§Y›B*CJphhàe¹h§Y›0J5B*\phhàe¹h§Y›B*phhàe¹h§Y›B*H*ph'h!‹h§Y›0J5B*\mH phsH  h!‹h§Y›B*H*mH phsH h!‹h§Y›B*mH phsH ì‹ï‹ñ‹eŒfŒgŒsŒtŒˆŒ‰Œ›ŒœŒ²Œ³Œ´ŒµŒàŒáŒŽêŽîŽòŽöŽ£‘¥‘ˑӑؑߑ’ïâÓö«ž«ž«ž«žŽž«ž«ŽrcrcrrTrTrhàe¹h§Y›6B*CJphhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJphhàe¹h§Y›B*CJH*phhàe¹h§Y›0J5B*\phhàe¹h§Y›B*H*phhàe¹h§Y›B*phhàe¹h§Y›0JB*phjhàe¹h§Y›B*Uphhàe¹h§Y›0J5B*phhàe¹h§Y›0JB*phhàe¹h§Y›0J56B*ph’ ’!’†’‡’ˆ’‹’’»’¼’½’Βϒã’ä’ñ’ô’õ’ö’f“g“±“²“íÝÒÝŵŦݙŠyŠyŠyeXÒXÒHhàe¹h§Y›0J5B*\phhàe¹h§Y›B*H*ph'hàe¹h§Y›0J5B*\mHphsH hàe¹h§Y›B*H*mHphsHhàe¹h§Y›B*mHphsHhàe¹h§Y›0JB*phhàe¹h§Y›0J5B*phhàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phhàe¹h§Y›B*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\ph½’õ’²“ò•Ŗ—¡—Ù§šؚ-›ž8ž¯žFŸå¢»£6¤e¥ΧQ¨~¨·¨i«ñãÕÇñãÕÇñãÕÇñãÕÇñãÕÇñãÕgd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.gd§Y›lÆ#ÿ.²“̓Γғԓۓݓÿ”•ñ•ò•ó•Y–Z–[–^–`–£–¥–ÖĖŖۖܖã–ä–ú–û–——òãòãòãòÔò²§²šŠš{k{²^§Q§Q§Q§Qhàe¹h§Y›B*H*phhàe¹h§Y›0JB*phhàe¹h§Y›0J5B*H*phhàe¹h§Y›0J5B*phhàe¹h§Y›0J56B*phhàe¹h§Y›0JB*phhàe¹h§Y›B*phjhàe¹h§Y›B*Uph#hàe¹h§Y›0J5B*CJ\phhàe¹h§Y›6B*CJphhàe¹h§Y›B*CJH*phhàe¹h§Y›B*CJph———c—d— —¡—™Ùę*š+š,š/š1š¥š¦š§š¶š·ššÚ֚ךؚٚ,›-›žŸžžž
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