A widespread family of heat-resistant obscure (Hero ... - SciSpace
Proteins are typically denatured and aggregated by heat. Exceptions to this principle include highly disordered and heat-resistant proteins found in ...
1 Title A chaperone and a heat-resistant obscure (Hero) protein ...The proteins were snap frozen in aliquots and stored at ?80°C. Protein purification via genetic code expansion. Genetic code expansion was ... A widespread family of heat-resistant obscure (Hero) proteins ...Before the Hero plasmid transfection, the MNs were re-seeded into a. Matrigel-coated 96-well plate at a density of 2,000 cells/cm2 dissociated by Accutase ... A chaperone and a heat-resistant obscure (Hero) protein ... - bioRxivHEK293T cells were seeded and transfected after 24 h with pCAGEN plasmids in medium, EndoBCN-K (SiChem, SC-8014) supplemented, and harvested 48 ...
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